CD11c antibody mouse specific - HS-375 008

CD11c is a fibrinogen receptor involved in cell-cell interaction during inflammation

Rabbit monoclonal recombinant IgG

Cat. No.: HS-375 008

Amount: 50 µg

Price: $415.00

Cat. No. HS-375 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 2500 (see remarks) gallery IHC-P: 1 : 400 up to 1 : 2000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb322C12
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues surrounding AA 647 of mouse CD11c (UniProt Id: Q9QXH4)
Reactivity	Reacts with: mouse (Q9QXH4). No signal: human (P20702), rat (D3ZWZ1). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody SY-322C12. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet	hs-375_008.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-375 008

Amount: 50 µg

Price: $415.00

IHC

IHC-P

Background

CD11c or Integrin alpha-X (ITGAX) is a heterodimeric glycoprotein consisting of an α- and β-subunit and has seven repeating integrin domains. This transmembrane receptor type I plays a pivotal role in T cell killing and mediates intercellular adhesions during inflammation. Predominat expression levels have been found in dendritic cells, monocytes, macrophages, neutrophils and a small subset of B cells. Under pathological conditions, CD11c is a marker for hairy cell leukemia, acute non-lymphocytic leukemias, and some chronic lymphocytic leukemias.

1 ) Osugi Y et al., Blood (2002)
2 ) Marotta G et al., Leuk. Lymphoma (2000)
3 ) Córbi AL et al., Leuk. Lymphoma (1997)
4 ) López-Rodríguez C et al., Eur. J. Immunol. (1995)

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