CD31 antibody mouse specific - HS-351 117

CD31 is an endothelial cell marker

Rat monoclonal purified IgG

Cat. No.: HS-351 117

Amount: 100 µg

Price: $415.00

Cat. No. HS-351 117	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 1000 gallery IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-53A2
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to residues surrounding AA 680 of mouse CD31 (UniProt Id: Q08481)
Reactivity	Reacts with: mouse (Q08481), rat. No signal: human (P16284). Other species not tested yet.
Remarks	IHC-Fr: PFA fixation is recommended.
Data sheet	hs-351_117.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-351 117

Amount: 100 µg

Price: $415.00

IHC

IHC-P

IHC-Fr

Background

CD31 or platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a cell adhesion molecule that contains a single trans-membrane domain and 6 Ig-like C2-type (immunoglobulin-like) domains (1). It is expressed on the surface of leukocytes and platelets and is primarily concentrated at endothelial cell-cell junctions (2). CD31 plays a role in several important biological processes, such as vascular development, maintenance of vascular endothelial barrier function and leukocyte emigration at inflammatory sites. In addition, CD31 promotes cancer metastasis by inducing epithelial-mesenchymal transition (3).

1 ) Privratsky JR et al., Cell Tissue Res. (2014)
2 ) Lertkiatmongkol P et al., Curr Opin Hematol (2016)
3 ) Zhu X et al., Gut Liver (2021)

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