Synaptic Systems - Protein A Agarose

Cat. No.: AG-A1003

Amount: 3 ml

Price: $115.00

Background

As the developer of high quality antibodies, we have extended our product portfolio by Protein A Agarose affinity resins.
Protein A is a cell wall protein from Staphylococcus aureus and is commonly used for the affinity purification or pull downs of IgG class antibodies. It binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region. Antibodies from serum (polyclonal antibodies), ascites or cell culture supernatant of hybridoma cell lines (monoclonal antibodies) can thus be easily isolated.

Protein A does not bind to all IgG classes from all species equivalently well.

Species	Subclass	Protein A binding
Human	IgA	variable
	IgD	-
	IgE	-
	IgG1	++++
	IgG2	++++
	IgG3	-
	IgG4	++++
	IgM	variable
Avian egg yolk	IgY	-
Cow	IgG	++
Dog	IgG	++
Goat	IgG	-
Guinea pig	IgG1	++++
	IgG2	++++
Hamster	IgG	+
Horse	IgG	++
Llama	IgG	-
Monkey (rhesus)	IgG	++++
Mouse	IgG1	+
	IgG2a	++++
	IgG2b	+++
	IgG3	++
	IgM	-
Pig	IgG	+++
Rabbit	IgG1	++++
Rat	IgG1	-
	IgG2a	-
	IgG2b	-
	IgG3	+
Sheep	IgG	+/-

1 ) OEDING P et al., Acta Pathol Microbiol Scand (1964)
2 ) Forsgren A et al., J. Immunol. (1968)
3 ) Kronvall G et al., J. Immunol. (1970)
4 ) Lind I et al., Acta Pathol Microbiol Scand B Microbiol Immunol (1970)
5 ) Kronvall G et al., Immunochemistry (1970)
6 ) Grov A et al., Acta Pathol Microbiol Scand Suppl (1973)

Protocols

Other products for this target

Cat. No. AG-A1003	3ml 50% slurry
Storage	Store at 2-8 °C, do not freeze
Applications	IP: yes
Capacity (DBC 10%) (determined with 1 mg/ml hu IgG1 MAB using a 1 ml column).	≥70 mg hu IgG/ml resin at residence time 6 min ≥50 mg hu IgG/ml resin at residence time 2.4 min ≥25 mg hu IgG/ml resin at residence time 1 min
Flow rate	250 cm/h (50% suspension)
CIP (0.5 M NaOH 100 Cycles)	No significant change in performance after 100 cycles with 0.5 M NaOH (1 cycle = 15 min contact time) use at room temperature.
Particle size	45 µm
Formulation	50 % slurry in PBS containing 1mM EDTA, 0.01% azide
Shelf life	Stable for 6 months.
Data sheet	ag-a1003.pdf
Documents	MSDS.pdf

Protein A Agarose affinity resin - AG-A1003