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Membrane Protein Extraction Kit MemEx - 800-MXK

A kit for fast and easy extraction of membrane proteins from eucaryotic cells and tissues
Cat. No.: 800-MXK
Amount: 1
Price: 250.00 €
Cat. No. 800-MXK

Content
800-MXK A: SySy MemEx reagent A, 10 ml
800-MXK B: SySy MemEx reagent B, 30 ml
800-MXK C: SySy MemEx reagent C, 10 ml
800-MXK D: SySy MemEx dye, 300 µl.
Reagents are sufficient for 130 extractions of up to 300 µg protein.

Storage Product is shipped at ambient temperature. Store at 4°C upon receipt.
Reagent A appears cloudy at RT. Storage at 4°C will clarify the solution. Keep reagent A at 4°C or on ice at all times during usage of the kit.
Reagent C precipitates at 4°C and should be dissolved by warming before use.
Gallery
Membrane protein fractionation from
rat brain

blot
Quantification

Gallery
Membrane protein fractionation from
3T3 cells

blot
Quantification

Shelf life 6 months
Documents Instruction manual
MSDS
Cat. No.: 800-MXK
Quantity: 1
Price: 250.00 €
Background

SySy MemEx employs a detergent-based temperature-dependent phase separation to enrich membrane proteins from mammalian tissue or cell homogenates. By this simple and rapid procedure you obtain a rather pure, non-denatured membrane protein fraction from any chosen protein homogenate. Most membrane proteins partition to the detergent phase with 80-90% efficiency, while the soluble proteins generally remain in the aqueous phase.
Depending on the starting material, the membrane proteins in the detergent phase will constitute 15-25% of input protein, thus being separated from many contaminating soluble proteins and other water-soluble compounds. Proteins with a very low expression level like Cellubrevin may become accessible to analysis only after enrichment. Abundant proteins like Tubulin or Synaptotagmin 1 can be found in both phases and may need a second round of extraction for complete purification. However, each protein shows individual behaviour depending on hydrophobicity and interactions with other (hydrophobic) proteins.
The membrane proteins isolated by SySy MemEx Kit are suitable for downstream applications like activity assays, immunoprecipitation, proteomic analysis or SDS-PAGE/western blotting. If the detergent should interfere with analysis, it can be removed by protein precipitation (denaturing) or dialysis against 0.5 % CHAPS in a suitable buffer (native; CHAPS can later be exchanged with other detergents).


Protocols
OTHER