Tailor-made Antibodies
and Tools for Life Science

mCLING - 710 006DY654

mCling is a fixable membrane probe for high resolution microscopy
Cat. No.: 710 006DY654
Amount: 5 nmol
Price: $360.00
Cat. No. 710 006DY654

5nmol mCLING labeled with DY® 654 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.

Applications
 
ICC: 1 : 50 up to 1 : 250 (1 - 0.2 nmol/ml) gallery  
IHC: 1 : 25 up to 1 : 50 (2 - 1 nmol/ml)
Label DY 654
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.

Data sheet 710_006dy654.pdf

References for mCLING - 710 006DY654

CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 710 006DY654 ICC; tested species: mouse
Cat. No.: 710 006DY654
Amount: 5 nmol
Price: $360.00