Tailor-made Antibodies
and Tools for Life Science

mCLING - 710 006AT488

mCling is a fixable membrane probe for high resolution microscopy
Cat. No.: 710 006AT488
Amount: 5 nmol
Price: $365.00
Cat. No. 710 006AT488

5nmol mCLING labeled with ATTO® 488 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.

 

Applications
 
ICC: 1 : 50 up to 1 : 250 (1 - 0.2 nmol/ml) gallery  
IHC: 1 : 25 up to 1 : 50 (2 - 1 nmol/ml)
Label ATTO 488
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.

Data sheet 710_006at488.pdf

References for mCLING - 710 006AT488

A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT488 ICC, FACS
HIV-1 Capsid Uncoating Is a Multistep Process That Proceeds through Defect Formation Followed by Disassembly of the Capsid Lattice.
Gifford LB, Melikyan GB
ACS nano (2024) 184: 2928-2947. 710 006AT488 ICC; tested species: human
Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).
Damstra HGJ, Mohar B, Eddison M, Akhmanova A, Kapitein LC, Tillberg PW
eLife (2022) 11: . 710 006AT488 ICC; tested species: human
Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis.
Shin W, Ge L, Arpino G, Villarreal SA, Hamid E, Liu H, Zhao WD, Wen PJ, Chiang HC, Wu LG
Cell (2018) : . 710 006AT488 ICC
Cat. No.: 710 006AT488
Amount: 5 nmol
Price: $365.00