Synaptic Systems - Paralemmin1

Cat. No.: 559 103

Amount: 50 µg

Price: $380.00

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Cat. No. 559 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P (FFPE): 1 : 2000 up to 1 : 10000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to the N-terminal half of mouse paralemmin1 (UniProt Id: Q9Z0P4-2)
Reactivity	Reacts with: mouse (Q9Z0P4-2), rat (Q920Q0), human (O75781-2). Other species not tested yet.
Specificity	K.O. validated PubMed: 40053592
Data sheet	Data Sheet 559_103 Safety Data Sheet (SDS)

References for Paralemmin1 - 559 103

Paralemmin-1, a modulator of filopodia induction is required for spine maturation.
Arstikaitis P, Gauthier-Campbell C, Carolina Gutierrez Herrera R, Huang K, Levinson JN, Murphy TH, Kilimann MW, Sala C, Colicos MA, El-Husseini A
Molecular biology of the cell (2008) 195: 2026-38. 559 103 WB, ICC; tested species: mouse,rat

Cellular and subcellular localization of paralemmin-1, a protein involved in cell shape control, in the rat brain, adrenal gland and kidney.
Kutzleb C, Petrasch-Parwez E, Kilimann MW
Histochemistry and cell biology (2007) 1271: 13-30. 559 103 WB, IHC, EM; tested species: rat

Paralemmin, a prenyl-palmitoyl-anchored phosphoprotein abundant in neurons and implicated in plasma membrane dynamics and cell process formation.
Kutzleb C, Sanders G, Yamamoto R, Wang X, Lichte B, Petrasch-Parwez E, Kilimann MW
The Journal of cell biology (1998) 1433: 795-813. 559 103 WB, ICC, EM

Paralemmin-1 controls the nanoarchitecture of the neuronal submembrane cytoskeleton.
Macarrón-Palacios V, Hubrich J, do Rego Barros Fernandes Lima MA, Metzendorf NG, Kneilmann S, Trapp M, Acuna C, Patrizi A, D'Este E, Kilimann MW
Science advances (2025) 1110: eadt3724. 559 103 ICC; KO verified; tested species: mouse

Cat. No.: 559 103

Amount: 50 µg

Price: $380.00

Immunofluorescence image of a rat hippocampus section stained for Paralemmin1 (red), NeuN (green), and Prox1 (white)

WB
(2 img)

ICC
(2 img)

IHC
(4 img)

IHC-P (FFPE)
(7 img)

Background

Paralemmin-1 (PALM1) is an alternatively spliced phosphoprotein of the paralemmin family, defined by a conserved paralemmin motif and a C-terminal prenylation/di-palmitoylation cysteine cluster (CaaX-like anchor) that targets the protein to the cytoplasmic face of membranes.
Paralemmin1 belongs to the Paralemmin family comprising four members (paralemmin1, ‑2, ‑3 and palmdelphin), which share a conserved domain structure. Palmdelphin is lacking the C‑terminal lipid anchor (3).
Functionally, Paralemmin-1 acts as a membrane-shape regulator linked to plasma-membrane dynamics, filopodia/process formation, dendritic spine maturation, and receptor-associated signaling (1, 2, 4). It localizes in discontinuous patches at the plasma membrane and endomembranes, prominently in neuronal perikarya, axons, dendrites, dendritic spines, and also in glial processes. Polarized membrane localization is seen in kidney and adrenal epithelia (1, 3). Paralemmin-1 expression is highest in brain, but also occurs in kidney, adrenal gland, lens fiber cells, retina, and lymphatic endothelial cells (1, 3, 5). Pathologically, increased paralemmin-1 is associated with breast cancer and contributes to tumor lymphangiogenesis (4, 5).

1 ) Kutzleb C et al., J Cell Biol (1998)
2 ) Arstikaitis P et al., Mol Biol Cell (2008)
3 ) Kutzleb C et al., Histochem Cell Biol (2007)
4 ) Turk CM et al., Cancer Cell Int (2012)
5 ) Albrecht I et al., Angiogenesis (2013)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Paralemmin1 (PALM1) antibody - 559 103 K.O.

References for Paralemmin1 - 559 103