Synaptic Systems

Cat. No.: 526 008

Amount: 50 µg

Price: $420.00

WB

ICC

IHC

IHC-P

Background

The Neural Cell Adhesion Molecule L1 (NCAM-L1), also known as L1CAM or CD171, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. Structurally, L1CAM comprises six immunoglobulin-like domains followed by five fibronectin type III domains in its extracellular region, a single transmembrane segment, and a short cytoplasmic tail (1, 2).
L1CAM is predominantly expressed on the surface of neurons mediating neuron-neuron adhesion and neurite outgrowth (3). It is also found in non-neuronal cells such as immature oligodendrocytes, Schwann cells, and certain immune cells like T cells (4, 5). Moreover, L1CAM expression has been observed in a variety of tumors across different organ systems (6).
Mutations or dysregulation of L1CAM are associated with several neurological disorders collectively termed L1 syndrome. These comprise X-linked hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait, and adducted thumbs), and spastic paraplegia type 1 (7).

1 ) Moos M et al., Nature (1988)
2 ) Chen L et al., Dev Dyn (2010)
3 ) Loers G et al., Int J Mol Sci (2023)
4 ) Wood PM et al., J Neurosci (1990)
5 ) Grage-Griebenow E et al., Mol Oncol (2014)
6 ) Inaguma S et al., Oncotarget (2016)
7 ) Zhou H et al., Front Genet (2022)
8 ) Rosenthal A et al., Nat Genet (1992)

Protocols

Cat. No. 526 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 gallery
Clone	Rb557B6
Subtype	IgG1 (κ light chain)
Immunogen	Protein complex consisting of L1CAM cross-linked with a monoclonal antibody to the L2/HNK-1 carbohydrate epitope and KLH (UniProt Id: P11627)
Reactivity	Reacts with: mouse (P11627). No signal: rat. Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody 557B6. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. WB: Due to its large size, L1CAM requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with NuPAGE 3-8% TRIS-Acetate gradient gels from Invitrogen.
Data sheet	526_008.pdf

L1CAM (NCAM-L1) antibody - 526 008