Synaptic Systems

Cat. No.: 524 005

Amount: 50 µg

Price: $465.00

WB

ICC

IHC

IHC-P

Background

LRBA (Lipopolysaccharide-Responsive and Beige-Like Anchor Protein) is a member of the BEACH-protein family. It is a large cytosolic protein comprising several functional domains, including the Plekstrin Homology (PH)-like domain, BEACH domain, and multiple WD40 repeats (1).
LRBA is essential for the proper functioning of immune cells, particularly B cells and T cells. It has been shown to regulate the recycling of the immune receptor CTLA-4, which is crucial for T cell activation and downregulation (2, 3). LRBA deficiency manifests as immunodeficiency, autoimmune diseases, and various hematological disorders, like reduced levels of B cells, impaired immunoglobulin production and more (4).
Dysfunctional LRBA is also connected to several CNS disorders like sensorineural hearing loss (5), cerebral granulomatous lesions, nerve demyelination and atrophy (6).
LRBA is widely expressed in multiple tissues including lymphoid organs, the gastrointestinal tract, kidney, the pancreas, and neuronal cells (7).

1 ) Cullinane AR et al., Traffic (2013)
2 ) Lo B et al., Science (2015)
3 ) Burnett DL et al., Immunol Cell Biol (2017)
4 ) Alkhairy OK et al., J Clin Immunol (2016)
5 ) Vogl C et al., EMBO Rep (2017)
6 ) Mangodt TC et al., BMC Pediatr (2023)
7 ) Roussa E et al., Sci Rep (2024)

Protocols

Other products for this target

Cat. No. 524 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 1000 gallery IHC-P: 1 : 1000 gallery
Immunogen	Recombinant protein corresponding to residues near the central region of mouse LRBA (UniProt Id: Q9ESE1)
Reactivity	Reacts with: mouse (Q9ESE1). Other species not tested yet.
Remarks	WB: Due to its large size, LRBA requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with NuPAGE 3-8% TRIS-Acetate gradient gels from Invitrogen. Unboiled samples are recommended.
Data sheet	524_005.pdf

LRBA antibody - 524 005