Synaptic Systems

Cat. No.: 519 005

Amount: 50 µg

Price: $465.00

WB

IHC-G

Background

LAT1 is ubiquitously expressed, with highest levels observed in the brain, spleen, bone marrow, testis, and placenta. In the blood-brain barrier, LAT1 is localized on both apical and basolateral membranes. In other polarized epithelia, it is mainly localized in basolateral membranes. In the placenta, LAT1 is present on both, the maternal and fetal surfaces of the syncytiotrophoblasts (1, 2).
Like all members of the heteromeric amino acid transporter (HAT, SLC7) family, LAT1 resides in the plasma membrane in a heterodimeric form. The LAT1 holotransporter consists of a 55-kDa light chain, SCL7A5 (LAT1 proper), and an escort protein called the heavy chain covalently linked to the light chain via a disulfide bond. LAT1 heterodimerizes with the 4F2hc (SLC3A2) heavy chain, an N-glycosylated ~68-kD transmembrane protein with one membrane-spanning domain. While transport carried out by either chain alone is negligible, the heavy chain is only needed to stabilize the dimer and facilitate its translocation to the plasma membrane, and the actual transport is carried out by the light chain (3).
LAT1 takes part in the transport of a wide range of neutral amino acids, especially ones with large branched or aromatic side chains. Tryptophan, phenylalanine, leucine, and histidine are transported with high affinity. Glutamine as an uptake substrate has a low affinity toward LAT1. Histidine and tyrosine are transported bidirectionally, whereas the others are preferentially transported in the inward direction only. LAT1 displays asymmetrical affinity towards bidirectionally transported substrates, with extracellular versus intracellular Km values being in the micromolar versus millimolar range (4). The generally accepted mode of function of LAT1 is obligatory antiport, i.e. the exchange of a large and neutral extracellular substrate for an abundant intracellular amino acid such as glutamine (5).

1 ) Fotiadis D et al., Mol Aspects Med (2013)
2 ) Scalise M et al., Front Chem (2018)
3 ) Yanagida O et al., Biochim Biophys Acta (2001)
4 ) Meier C et al., EMBO J (2002)
5 ) Singh N et al., Int J Mol Sci (2018)

Protocols

Cat. No. 519 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet IHC-G: 1 : 1000 (see remarks) gallery
Immunogen	Recombinant protein corresponding to residues near the amino terminus of rat LAT1 (UniProt Id: Q63016)
Reactivity	Reacts with: human (Q01650), rat (Q63016), mouse (Q9Z127). Other species not tested yet.
Remarks	IHC-G: 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended
Data sheet	519_005.pdf

LAT1 (SLC7A5) antibody - 519 005