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BCRP1/ABCG2 antibody - 518 005

BCRP1/ABCG2 is a member of the ATP-binding cassette transporter family
Guinea pig polyclonal purified antibody
Cat. No.: 518 005
Amount: 50 µg
Price: $460.00
Cat. No. 518 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
ICC: 1 : 1000 gallery  
IHC: not tested yet
IHC-P: 1 : 500 up to 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to residues near the carboxy region of mouse BCRP1/ABCG2 (UniProt Id: Q7TMS5)
Reactivity Reacts with: mouse (Q7TMS5).
Weaker signal: rat (Q80W57).
Other species not tested yet.
Data sheet 518_005.pdf
Cat. No.: 518 005
Amount: 50 µg
Price: $460.00
Background

BCRP1/ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane-spanning regions, suggesting a function as a semi-transporter (1).
Neurospheres have been shown to have high levels of BCRP1/ABCG2, and approximately 63% of cells in neurospheres were BCRP1/ABCG2-positive, similar to the proportion of nestin-positive cells, and in most cases BCRP1/ABCG2 and nestin staining were colocalized in the same cells (2). BCRP1/ABCG2 was originally discovered in a multidrug-resistant breast cancer cell line. Past studies have expanded the understanding of its role in physiology, disease pathology, and drug resistance (3). Physiologically, BCRP1/ABCG2 is expressed in barrier organs and tissues including the blood-brain barrier, where BCRP1/ABCG2 localizes at the luminal plasma membrane of endothelial cells and facilitates directional efflux across the blood-brain barrier from brain to blood (4, 5).