Synaptic Systems

Cat. No.: 518 005

Amount: 50 µg

Price: $465.00

WB

ICC

IHC-P

Background

BCRP1/ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane-spanning regions, suggesting a function as a semi-transporter (1).
Neurospheres have been shown to have high levels of BCRP1/ABCG2, and approximately 63% of cells in neurospheres were BCRP1/ABCG2-positive, similar to the proportion of nestin-positive cells, and in most cases BCRP1/ABCG2 and nestin staining were colocalized in the same cells (2). BCRP1/ABCG2 was originally discovered in a multidrug-resistant breast cancer cell line. Past studies have expanded the understanding of its role in physiology, disease pathology, and drug resistance (3). Physiologically, BCRP1/ABCG2 is expressed in barrier organs and tissues including the blood-brain barrier, where BCRP1/ABCG2 localizes at the luminal plasma membrane of endothelial cells and facilitates directional efflux across the blood-brain barrier from brain to blood (4, 5).

1 ) Krishnamurthy P et al., Annu Rev Pharmacol Toxicol (2006)
2 ) Scharenberg CW et al., Blood (2002)
3 ) Kukal S et al., Cell Mol Life Sci (2021)
4 ) Cooray HC et al., Neuroreport (2002)
5 ) Eisenblätter T et al., Biochem Biophys Res Commun (2002)

Protocols

WB

ICC

Fixed Cells

IHC-P

Cat. No. 518 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery ICC: 1 : 1000 gallery IHC: not tested yet IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy region of mouse BCRP1/ABCG2 (UniProt Id: Q7TMS5)
Reactivity	Reacts with: mouse (Q7TMS5). Weaker signal: rat (Q80W57). Other species not tested yet.
Data sheet	Datasheet 518_005

BCRP1 (ABCG2) antibody - 518 005