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NP-EGTA - 510 006

A photolabile Ca2+ chelator
Cat. No.: 510 006
Amount: 1 mg
Price: 390.00 €
Cat. No. 510 006

1 mg o-nitrophenyl EGTA tetrapotassium salt dissolved in 50 µl H2O (= 30.5 mM). HPLC analysis
Spin down and store at -20° C. Protect material from light always.

Name

6,9-Dioxa-3,12-diazatetradecanedioic acid, 3,12-bis(carboxymethyl)-4-(2-nitrophenyl)-tetrapotassium salt.

Molecular formula

C20H23K4N3O12. chemical structure

Molecular weight

653.81

Extinction coefficient

ε = 5.52 x 103 M-1 x cm-1 at 260 nm in Ca2+ free 40 mM HEPES / 100 mM KCl buffer at pH 7.2.

Photolysis quantum yield

0.23

Documents MSDS
Data sheet 510_006.pdf

References for NP-EGTA - 510 006

Phosphatidylinositol 4,5-bisphosphate optical uncaging potentiates exocytosis.
Walter AM, Müller R, Tawfik B, Wierda KD, Pinheiro PS, Nadler A, McCarthy AW, Ziomkiewicz I, Kruse M, Reither G, Rettig J, et al.
eLife (2017) 6: . 510 006
Synaptotagmin interaction with SNAP-25 governs vesicle docking, priming, and fusion triggering.
Mohrmann R, de Wit H, Connell E, Pinheiro PS, Leese C, Bruns D, Davletov B, Verhage M, Sørensen JB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3336: 14417-30. 510 006
Doc2b synchronizes secretion from chromaffin cells by stimulating fast and inhibiting sustained release.
Pinheiro PS, de Wit H, Walter AM, Groffen AJ, Verhage M, Sørensen JB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16459-70. 510 006
Analysis of neurotransmitter release mechanisms by photolysis of caged Ca²⁺ in an autaptic neuron culture system.
Burgalossi A, Jung S, Man KN, Nair R, Jockusch WJ, Wojcik SM, Brose N, Rhee JS
Nature protocols (2012) 77: 1351-65. 510 006
Rab3 proteins involved in vesicle biogenesis and priming in embryonic mouse chromaffin cells.
Schonn JS, van Weering JR, Mohrmann R, Schlüter OM, Südhof TC, de Wit H, Verhage M, Sørensen JB
Traffic (Copenhagen, Denmark) (2010) 1111: 1415-28. 510 006
Fast vesicle fusion in living cells requires at least three SNARE complexes.
Mohrmann R, de Wit H, Verhage M, Neher E, Sørensen JB
Science (New York, N.Y.) (2010) 3306003: 502-5. 510 006
SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming.
Burgalossi A, Jung S, Meyer G, Jockusch WJ, Jahn O, Taschenberger H, O'Connor VM, Nishiki T, Takahashi M, Brose N, Rhee JS, et al.
Neuron (2010) 683: 473-87. 510 006
Progression of diet-induced diabetes in C57BL6J mice involves functional dissociation of Ca2(+) channels from secretory vesicles.
Collins SC, Hoppa MB, Walker JN, Amisten S, Abdulkader F, Bengtsson M, Fearnside J, Ramracheya R, Toye AA, Zhang Q, Clark A, et al.
Diabetes (2010) 595: 1192-201. 510 006
Role of the synaptobrevin C terminus in fusion pore formation.
Ngatchou AN, Kisler K, Fang Q, Walter AM, Zhao Y, Bruns D, Sørensen JB, Lindau M
Proceedings of the National Academy of Sciences of the United States of America (2010) 10743: 18463-8. 510 006
Cat. No.: 510 006
Quantity: 1 mg
Price: 390.00 €
Phosphatidylinositol 4,5-bisphosphate optical uncaging potentiates exocytosis.
Walter AM, Müller R, Tawfik B, Wierda KD, Pinheiro PS, Nadler A, McCarthy AW, Ziomkiewicz I, Kruse M, Reither G, Rettig J, et al.
eLife (2017) 6: . 510 006
Synaptotagmin interaction with SNAP-25 governs vesicle docking, priming, and fusion triggering.
Mohrmann R, de Wit H, Connell E, Pinheiro PS, Leese C, Bruns D, Davletov B, Verhage M, Sørensen JB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3336: 14417-30. 510 006
Doc2b synchronizes secretion from chromaffin cells by stimulating fast and inhibiting sustained release.
Pinheiro PS, de Wit H, Walter AM, Groffen AJ, Verhage M, Sørensen JB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16459-70. 510 006
Analysis of neurotransmitter release mechanisms by photolysis of caged Ca²⁺ in an autaptic neuron culture system.
Burgalossi A, Jung S, Man KN, Nair R, Jockusch WJ, Wojcik SM, Brose N, Rhee JS
Nature protocols (2012) 77: 1351-65. 510 006
Rab3 proteins involved in vesicle biogenesis and priming in embryonic mouse chromaffin cells.
Schonn JS, van Weering JR, Mohrmann R, Schlüter OM, Südhof TC, de Wit H, Verhage M, Sørensen JB
Traffic (Copenhagen, Denmark) (2010) 1111: 1415-28. 510 006
Fast vesicle fusion in living cells requires at least three SNARE complexes.
Mohrmann R, de Wit H, Verhage M, Neher E, Sørensen JB
Science (New York, N.Y.) (2010) 3306003: 502-5. 510 006
SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming.
Burgalossi A, Jung S, Meyer G, Jockusch WJ, Jahn O, Taschenberger H, O'Connor VM, Nishiki T, Takahashi M, Brose N, Rhee JS, et al.
Neuron (2010) 683: 473-87. 510 006
Progression of diet-induced diabetes in C57BL6J mice involves functional dissociation of Ca2(+) channels from secretory vesicles.
Collins SC, Hoppa MB, Walker JN, Amisten S, Abdulkader F, Bengtsson M, Fearnside J, Ramracheya R, Toye AA, Zhang Q, Clark A, et al.
Diabetes (2010) 595: 1192-201. 510 006
Role of the synaptobrevin C terminus in fusion pore formation.
Ngatchou AN, Kisler K, Fang Q, Walter AM, Zhao Y, Bruns D, Sørensen JB, Lindau M
Proceedings of the National Academy of Sciences of the United States of America (2010) 10743: 18463-8. 510 006
Background
Calcium is the most important signalling molecule inside cells. It is involved in the regulation of neurotransmission, gene-expression, muscle contraction and many more.
o-nitrophenyl EGTA (NP-EGTA) is a photolabile Ca2+ chelator that is highly specific for Ca2+ ions and unaffected by physiological Mg2+ concentrations. Photolysis by illumination with UV-light decreases the affinity of NP-EGTA for Ca2+ ions ~ 12,500-fold and the Ca2+ ions become physiologically available. By this approach regulatory effects of calcium on cellular processes can be studied.