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Brain lysate - 506-TOT

Ready to use tissue lysate for western blots
Cat. No.: 506-TOT
Amount: 200 µg
Price: $280.00
Cat. No. 506-TOT 200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'.
Total volume: 100 µl. Concentration: 2 mg/ml.
Aliquot and store at -20°C until use.
Applications WB: yes
Documents MSDS
Loading/lane 10 µg
Source Balb-C
Remarks

Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol.

Data sheet 506-tot.pdf
Cat. No.: 506-TOT
Quantity: 200 µg
Price: $280.00
Background

In many cases the relative concentration of the protein of interest is high enough to use whole brain lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. Fractions have been prepared according to our protocol. Protein concentration has been determined with a Bradford protein quantification assay.