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Home > Products > 505 005

Tight junction protein ZO-3 antibody - 505 005

ZO-3 is a member of the MAGUK protein family
Guinea pig polyclonal purified antibody
Cat. No.: 505 005
Amount: 50 µg
Price: $460.00
Cat. No. 505 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 1000 (see remarks) gallery  
IHC-P: 1 : 1000 up to 1 : 2000 gallery  
IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse Tight junction protein ZO-3 (UniProt Id: Q9QXY1)
Reactivity Reacts with: mouse (Q9QXY1), rat (D3Z8G7), dog (O62683).
Other species not tested yet.
Remarks

ICC: Methanol fixation is recommended
IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining.
IHC-Fr: Methanol-acetone (1:1 mix) fixation is recommended.
Data sheet 505_005.pdf
Cat. No.: 505 005
Amount: 50 µg
Price: $460.00
WB
ICC
IHC
IHC
IHC-P
IHC-P
IHC-Fr
IHC-Fr
Background

ZO-3 is a 130 kDa protein identified by co-immunoprecipitation with ZO-1 from Madin-Darby canine kidney (MDCK) cells. (1)
ZO-3 is a member of the membrane-associated guanylate kinase-like protein (MAGUK) family associated with intracellular junctions. (2)
The zona occludens proteins ZO-1, -2 and -3 (also known as TJP1, 2 and 3) are peripheral membrane adaptor proteins that link transmembrane junction proteins such as occludin and claudin to the actin cytoskeleton (3).