Synaptic vesicle lysate - 504-LP2

Ready to use tissue lysates for western blots

Rat tissue lysate

Cat. No.: 504-LP2

Amount: 50 µg

Price: $285.00

Cat. No. 504-LP2	50 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 50 µl. Concentration: 1 mg/ml. Aliquot and store at -20°C until use.
Applications	WB: yes gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Loading/lane	10-30 µg
Source	Rat, White Wistar
Remarks	Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol.
Data sheet	Datasheet 504-lp2
Documents	MSDS

Cat. No.: 504-LP2

Amount: 50 µg

Price: $285.00

Background

In many cases the relative concentration of the protein of interest is high enough to use whole brain lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. Fractions have been prepared according to our protocol. Protein concentration has been determined with a Bradford protein quantification assay.

1 ) Koomoa DL et al., Neurosci. Lett. (2008)
2 ) Huttner WB et al., J. Cell Biol. (1983)
3 ) Nagy A et al., Brain Res. (1976)