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Brain lysate - 501-TOT

Ready to use tissue lysate for western blots
Cat. No.: 501-TOT
Amount: 200 µg
Price: $280.00
Cat. No. 501-TOT 200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'.
Total volume: 100 µl. Concentration: 2 mg/ml.
Aliquot and store at -20°C until use.
Applications WB: yes gallery  
Documents MSDS
Loading/lane 10-30 µg
Source White Wistar

Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol.

Data sheet 501-tot.pdf
Cat. No.: 501-TOT
Quantity: 200 µg
Price: $280.00

In many cases the relative concentration of the protein of interest is high enough to use whole brain lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. Fractions have been prepared according to our protocol. Protein concentration has been determined with a Bradford protein quantification assay.