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Home > Products > 490 011

APLP2 antibody - 490 011 K.O.

APLP2, a protein of the highly conserved amyloid precursor protein family
Mouse monoclonal purified IgG
Cat. No.: 490 011
Amount: 100 µg
Price: $415.00
Cat. No. 490 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-Staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone SY-158G12
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse APLP2 (UniProt Id: Q06335)
Reactivity Reacts with: mouse (Q06335), rat (P15943).
Other species not tested yet.
Specificity K.O. validated
Data sheet 490_011.pdf
Cat. No.: 490 011
Amount: 100 µg
Price: $415.00
WB
ICC
Background

Amyloid beta precursor like protein 1 and 2 (APLP1 and 2) and amyloid precursor protein (APP) belong to one protein family that all share considerable sequence homolgy in the amino- and carboxyl termini (1, 2). In contrast to APP, APLP1 and 2 do not contain the Abeta motif. Like APP, APLP1 and 2 are processed by alpha-, beta-, and gamma-secretase leading to a secreted ecto-domain and a membrane-anchored C-terminal fragment (3). APLPs function as synaptic adhesion molecules (SAMs) and are involved in synaptogenesis (4).
In contrast to the ubiquitously expressed APLP2, APLP1 selectively occurs in neuronal cells (4).