APLP2 antibody - 490 011 K.O.

APLP2, a protein of the highly conserved amyloid precursor protein family

Mouse monoclonal purified IgG

Cat. No.: 490 011

Amount: 100 µg

Price: $420.00

Cat. No. 490 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-158G12
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse APLP2 (UniProt Id: Q06335)
Reactivity	Reacts with: mouse (Q06335), rat (P15943). Other species not tested yet.
Specificity	K.O. validated
Data sheet	Datasheet 490_011

Cat. No.: 490 011

Amount: 100 µg

Price: $420.00

ICC

Background

Amyloid beta precursor like protein 1 and 2 (APLP1 and 2) and amyloid precursor protein (APP) belong to one protein family that all share considerable sequence homolgy in the amino- and carboxyl termini (1, 2). In contrast to APP, APLP1 and 2 do not contain the Abeta motif. Like APP, APLP1 and 2 are processed by alpha-, beta-, and gamma-secretase leading to a secreted ecto-domain and a membrane-anchored C-terminal fragment (3). APLPs function as synaptic adhesion molecules (SAMs) and are involved in synaptogenesis (4).
In contrast to the ubiquitously expressed APLP2, APLP1 selectively occurs in neuronal cells (4).

1 ) Sprecher CA et al., Biochemistry (1993)
2 ) Slunt HH et al., J Biol Chem (1994)
3 ) Li Q et al., J Biol Chem (2004)
4 ) Schilling S et al., J. Neurosci. (2017)

Protocols

ICC

Fixed Cells