Tailor-made Antibodies
and Tools for Live Science
Home|||||||Technical Support|

P2Y12 antibody - 476 011

P2Y12 is a Gi - coupled purinergic receptor relevant for microglia activation and platelet aggregation.
Mouse monoclonal purified IgG
Cat. No.: 476 011
Amount: 100 µg
Price: $415.00
Cat. No. 476 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1:1000 up to 1 : 1:10000 gallery  
IHC-P: 1 : 1:1000 up to 1 : 1:10000 gallery  
Clone SY-341D12
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of ms P2Y12 receptor (UniProt Id: Q9CPV9)
Reactivity Reacts with: mouse (Q9CPV9).
No signal: rat (Q9EPX4), human (Q9H244).
Other species not tested yet.
Specificity Specific for P2Y12
Data sheet 476_011.pdf
Cat. No.: 476 011
Amount: 100 µg
Price: $415.00
Background

P2Y12 receptor (P2RY12) is a Gi – coupled purinoceptor and is of particular relevance for microglia in the central nervous system (CNS) (1). The preferred agonist is ADP, the degradation product of ATP, which is released from neurons and other glial cells during physiological activity or after tissue damage. P2Y12 receptor is highly expressed in processes and somata of surveilling microglia and plays a major role in microglial chemotaxis in response to local CNS injury (2). More recently, P2Y12 receptors have been shown to be concentrated at microglia process-neuronal somata contacts (3) and to be critical for neuroprotection. To date, P2Y12 receptor is one of the most accepted microglia-specific markers used to distinguish CNS-resident microglia from CNS-associated macrophages (CAMs) and infiltrating monocytes/macrophages (4). The expression level of P2Y12 receptor is downregulated in an activated state of microglia referred to as disease-associated microglia (DAM). In the periphery, P2Y12 receptor is expressed in platelets and is a well-known biological target for anti-thrombotic drugs due to its central role in platelet activation, aggregation and blood clotting (5).