Tailor-made Antibodies
and Tools for Life Science

Collagen type IV antibody - 462 017

Collagen type IV is a basement membrane protein
Rat monoclonal purified IgG
Cat. No.: 462 017
Amount: 100 µg
Price: $420.00
Cat. No. 462 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not recommended
ICC: not tested yet
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery  
Clone SY-245D2
Subtype IgG2a (κ light chain)
Immunogen Human heterotrimeric Collagen type IV containing two α 1-like (UniProt Id: P02462) and one α 2-like (UniProt Id: P08572) chains.
Reactivity Reacts with: mouse (P02463), mouse (P08122), human (P02462), human (P08572).
No signal: rat (F1MA59), rat (F1M6Q3).
Other species not tested yet.
Remarks

IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C or 0.2 mg/ml pepsin in 0.2 M HCl for 20 min at 37 °C) are recommended
IHC-P: Antigen retrieval with Tris-EDTA buffer pH 9.0 in a food steamer followed by pepsin treatment or a Citrate buffer pH 6.0 antigen retrieval followed by pepsin treatment are recommended.
IHC-Fr: 4% FA, Methanol, Acetone and Methanol-acetone mix 1:1 fixation is possible

Data sheet 462_017.pdf
Cat. No.: 462 017
Amount: 100 µg
Price: $420.00