Tailor-made Antibodies
and Tools for Life Science

Collagen type IV antibody - 462 006

Collagen type IV is a basement membrane protein
Chicken polyclonal purified antibody
Cat. No.: 462 006
Amount: 50 µg
Price: $385.00
Cat. No. 462 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: yes (see remarks) gallery  
IHC-P: not tested yet
IHC-G: yes (see remarks) gallery  
Immunogen Human heterotrimeric Collagen type IV containing two α1-like (UniProt Id: P02462) and one α2-like (UniProt Id: P08572) chains
Reactivity Reacts with: mouse (P02463), mouse (P08122), human (P02462), human (P08572), rat (F1MA59), rat (F1M6Q3).
Other species not tested yet.
Remarks

IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) or an antigen retrieval with 0.2 mg/ml pepsin in 0.2 M HCl for 20 min at 37 °C are required for immunohistochemical staining
IHC-G: 3% glyoxal, 1% acetic acid, 20% ethanol, in ddH2O, pH 4.2-4.4, according to Richter et al. 2017 is recommended

Data sheet 462_006.pdf
Cat. No.: 462 006
Amount: 50 µg
Price: $385.00