|Cat. No. 447 005||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 2000 gallery
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery
IHC-P/FFPE: 1 : 200 up to 1 : 500 gallery
|Immunogen||Recombinant protein corresponding to AA 264 to 521 from mouse Occludin (UniProt Id: Q61146)|
Reacts with: mouse (Q61146), rat (Q6P6T5), dog (Q28269).
Other species not tested yet.
|Specificity||Specific for Occludin|
ICC: Methanol fixation is recommended
IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended
Occludin, a 65-kDa tetraspan integral membrane protein, plays a role in the formation and regulation of the tight junction paracellular permeability barrier. The C-terminal is cytoplasmic and is important for interaction with ZO-1, which links the protein complex to the actin cytoskeleton. Occludin is localized at tight junctions of both epithelial and endothelial cells. (1) It is highly expressed in the testis, kidney, lung, liver and brain. Expression of occludin is required for the infection of liver cells by the hepatitis C virus (HCV). (2)