Tailor-made Antibodies
and Tools for Life Science

Occludin antibody - 447 005

Occludin is an integral membrane protein located at the tight junction
Guinea pig polyclonal purified antibody
Cat. No.: 447 005
Amount: 50 µg
Price: $465.00
Cat. No. 447 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P: 1 : 200 up to 1 : 500 gallery  
IHC-Fr: 1 : 500 (see remarks) gallery  
Immunogen Recombinant protein corresponding to AA 264 to 521 from mouse Occludin (UniProt Id: Q61146)
Reactivity Reacts with: mouse (Q61146), rat (Q6P6T5), dog (Q28269).
Other species not tested yet.
Remarks

ICC: Methanol fixation is recommended
IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended
IHC-Fr: PFA and Acetone fixation are recommended. Methanol fixation is possible.

Data sheet 447_005.pdf

References for Occludin - 447 005

Vascular Development of Fetal and Postnatal Neocortex of the Pig, the European Wild Boar Sus scrofa.
Sobierajski E, Czubay K, Beemelmans C, Beemelmans C, Meschkat M, Uhlenkamp D, Meyer G, Wahle P
The Journal of comparative neurology (2024) 53212: e70011. 447 005 WB; tested species: pig
Cat. No.: 447 005
Amount: 50 µg
Price: $465.00