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Home > Products > 447 005

Occludin antibody - 447 005

Occludin is an integral membrane protein located at the tight junction
Guinea pig polyclonal purified antibody
Cat. No.: 447 005
Amount: 50 µg
Price: $460.00
Cat. No. 447 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P: 1 : 200 up to 1 : 500 gallery  
IHC-Fr: 1 : 500 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 264 to 521 from mouse Occludin (UniProt Id: Q61146)
Reactivity Reacts with: mouse (Q61146), rat (Q6P6T5), dog (Q28269).
Other species not tested yet.
Remarks

ICC: Methanol fixation is recommended
IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended
IHC-Fr: PFA and Acetone fixation are recommended. Methanol fixation is possible.
Data sheet 447_005.pdf
Cat. No.: 447 005
Amount: 50 µg
Price: $460.00
WB
ICC
IHC
IHC
IHC
IHC
IHC-P
IHC-Fr
IHC-Fr
IHC-Fr
IHC-Fr
IHC-Fr
Background

Occludin, a 65-kDa tetraspan integral membrane protein, plays a role in the formation and regulation of the tight junction paracellular permeability barrier. The C-terminal is cytoplasmic and is important for interaction with ZO-1, which links the protein complex to the actin cytoskeleton. Occludin is localized at tight junctions of both epithelial and endothelial cells. (1) It is highly expressed in the testis, kidney, lung, liver and brain. Expression of occludin is required for the infection of liver cells by the hepatitis C virus (HCV). (2)