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Occludin - 447 005

an integral membrane protein located at the tight junction
Polyclonal Guinea pig purified antibody
Cat. No.: 447 005
Amount: 50 µg
Price: 450.00 €
Cat. No. 447 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 2000 gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: 1 : 200 up to 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 264 to 521 from mouse Occludin (UniProt Id: Q61146)
Reactivity Reacts with: mouse (Q61146), rat (Q6P6T5), dog (Q28269).
Other species not tested yet.
Specificity Specific for Occludin
Remarks

ICC: Methanol fixation is recommended

IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended

Data sheet 447_005.pdf
Cat. No.: 447 005
Quantity: 50 µg
Price: 450.00 €
Background

Occludin, a 65-kDa tetraspan integral membrane protein, plays a role in the formation and regulation of the tight junction paracellular permeability barrier. The C-terminal is cytoplasmic and is important for interaction with ZO-1, which links the protein complex to the actin cytoskeleton. Occludin is localized at tight junctions of both epithelial and endothelial cells. (1) It is highly expressed in the testis, kidney, lung, liver and brain. Expression of occludin is required for the infection of liver cells by the hepatitis C virus (HCV). (2)