BCAS1 antibody - 445 308

BCAS1 is a protein specific for an actively myelinating oligodendrocyte subpopulation

Guinea pig monoclonal recombinant IgG

Cat. No.: 445 308

Amount: 50 µg

Price: $420.00

Cat. No. 445 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 2000 up to 1 : 5000 gallery IHC-P (FFPE): 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp203G7
Subtype	IgG2 (κ light chain)
Immunogen	Synthetic peptide corresponding to C-terminal residues of mouse BCAS1 (UniProt Id: Q80YN3)
Reactivity	Reacts with: mouse (Q80YN3). Weaker signal: rat (Q3ZB98). No signal: human (O75363-1). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody SY-203G7. The constant regions of the heavy and light chains have been replaced with guinea pig specific sequences. The antibody can therefore be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells. RUO: For this product an additional Limited Use Label License (LULL) for internal Research Use Only (RUO) from Life Technologies Corporation applies. The recombinant proteins/antibodies you will receive/have received from Synaptic Systems GmbH (“Materials“) were provided or produced under a license between Synaptic Systems GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies“) through the use of Life Technologies proprietary cell lines and expression products. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit, up to and including pharmacokinetic and toxicology studies required to meet the regulations for filing an IND. IND submissions, first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial purposes or applications of any kind, including, without limitation, manufacturing or quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008, United States.
Data sheet	Data Sheet 445_308 Safety Data Sheet (SDS)

Cat. No.: 445 308

Amount: 50 µg

Price: $420.00

Immunofluorescence image of a rat cortex section stained for BCAS1 (red) and Olig2 (green)

ICC
(1 img)

IHC
(6 img)

IHC-P (FFPE)
(6 img)

Background

Breast carcinoma-amplified sequence 1 (BCAS1) was originally identified as a potential oncogene amplified in human cancer cell lines (1). It is also highly expressed in brain (2) where it mainly defines an oligodendroglial subpopulation occuring at regions of active myelin formation (3).
In fetal brain BCAS1 positive oligodendrocytes are restricted to the white matter. In adult brains they persist in the grey matter until old age (3).

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Collins C et al., Proc. Natl. Acad. Sci. U.S.A. (1998)
2 ) Ishimoto T et al., Glia (2017)
3 ) Fard MK et al., Sci Transl Med (2017)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

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