Cat. No.: 435 004
Amount: 100 µl
Price:
$365.00
Cat. No. 435 004 |
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 500 gallery |
Immunogen | Recombinant protein corresponding to AA 1 to 1024 from E.coli β-galactosidase (UniProt Id: P00722) |
Specificity | Specific for β-Galactosidase. |
Data sheet | 435_004.pdf |
Galactosidases are enzymes that catalyze the hydrolysis of galactosides into monosaccharides. They can be classified as α- or β-Galactosidase. In E.coli the latter one is encoded by the Lac-Z gene and is responsible for breaking down the disaccharide lactose into its monosaccharide components, glucose and galactose.
β-Galactosidase can be used to track the efficiency of bacterial transformation with a recombinant plasmid in a process called Blue/White Color Screening. It is also a frequently used reporter gene to monitor tissue specific gene-expression.