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nNOS antibody - 432 005

nNos is a neuronal nitric oxide synthase
Guinea pig polyclonal purified antibody
Cat. No.: 432 005
Amount: 50 µg
Price: $465.00
Cat. No. 432 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 5000 up to 1 : 10000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 1416 to 1434 from human nNOS (UniProt Id: P29475)
Reactivity Reacts with: mouse (Q9Z0J4), rat (P29476), human (P29475).
Other species not tested yet.
Data sheet 432_005.pdf
Cat. No.: 432 005
Amount: 50 µg
Price: $465.00
Background

Neuronal nitric oxide synthase (nNOS, bNOS) is found in neurons of the central and peripheral nervous system, macula densa, and pancreatic islet cells. An alternative splice variant of nNos is specifically expressed in striated muscle. nNOS is soluble and predominantly found in the cytosol. It produces nitric oxide (NO), a messenger molecule with diverse functions and neurotransmitter like properties.