Saposin-D antibody - 431 002

SAP-D is a sphingolipid activator protein

Rabbit polyclonal antiserum

Cat. No.: 431 002

Amount: 200 µl

Price: $360.00

Cat. No. 431 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 410 to 487 from human Prosaposin (UniProt Id: P07602)
Reactivity	Reacts with: mouse (Q61207), human (P07602). Other species not tested yet.
Specificity	recognizes Saposin-D and Prosaposin
Data sheet	431_002.pdf

Cat. No.: 431 002

Amount: 200 µl

Price: $360.00

IHC

Background

The glycoprotein prosaposin is a precursor oft the sphingolipid activator proteins saposins A, B, C, and D also called (SAP-A, SAP-B, SAP-C and SAP-D). Prosaposin is targeted to lysosomes where it is proteolytically processed to the final SAP products.

Saposins are indispensable for lysosomal hydrolysis of sphingolipids and the lack of each saposin leads to a lysosomal storage disease. Sap-D-deficient mice accumulate ceramides with hydroxylated fatty acids mainly in brain and kidney.

1 ) Sandhoff K et al., Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2012)
2 ) Kolter T et al., Annu. Rev. Cell Dev. Biol. (2005)
3 ) Matsuda J et al., Hum. Mol. Genet. (2004)
4 ) Tyynelä J et al., Acta Neuropathol. (1995)

Protocols

IHC

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