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Home > Products > 429 011

Aquaporin4 antibody - 429 011

Aquaporin4 is a water-specific channel in the blood brain barrier
Mouse monoclonal purified IgG
Cat. No.: 429 011
Amount: 100 µg
Price: $415.00
Cat. No. 429 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 5000 gallery  
IHC-P: 1 : 1000 up to 1 : 2000 gallery  
IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 325B5A5
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 249 to 323 from mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity Reacts with: mouse (P55088-1), rat (P47863), human (P55087).
Other species not tested yet.
Specificity Specific for Aquaporin 4
Matching control protein/peptide 429-0P
Remarks

IHC-Fr: PFA fixation is recommended
Data sheet 429_011.pdf
Cat. No.: 429 011
Amount: 100 µg
Price: $415.00
ICC
ICC
IHC
IHC
IHC-P
IHC-P
IHC-P
IHC-P
IHC-Fr
IHC-Fr
Background

Aquaporin4, or AQP4, also known as Mercurial-insensitive water channel (MIWC), is a member of the aquaporin water channel family. This osmoreceptor regulates body water balance and mediates water flow within the central nervous system.
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