Synaptic Systems - Aquaporin4

Cat. No.: 429 011

Amount: 100 µg

Price: $420.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

Cat. No. 429 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 5000 (see remarks) gallery IHC-P (FFPE): 1 : 1000 up to 1 : 2000 gallery IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	325B5A5
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 249 to 323 from mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity	Reacts with: mouse (P55088-1), rat (P47863), human (P55087). Other species not tested yet.
Specificity	Specific for Aquaporin 4
Matching control protein/peptide	429-0P
Remarks	IHC: Antigen retrieval with pepsin is tolerated. IHC-Fr: 4% formaldehyde/PFA fixation is recommended.
Data sheet	Data Sheet 429_011 Safety Data Sheet (SDS)

References for Aquaporin4 - 429 011

Astroglial Hmgb1 regulates postnatal astrocyte morphogenesis and cerebrovascular maturation.
Freitas-Andrade M, Comin CH, Van Dyken P, Ouellette J, Raman-Nair J, Blakeley N, Liu QY, Leclerc S, Pan Y, Liu Z, Carrier M, et al.
Nature communications (2023) 141: 4965. 429 011 WB; tested species: mouse

Npc1 deficiency impairs brain vascular integrity in mouse model of Niemann-Pick disease type C1.
Kang J, Gao G, Qiao L, Xu X, Zhao Y, Xiao L, Cui C, Feng H, Liu Y, Salomoni P, Yan X, et al.
Neurobiology of disease (2026) 226: 107462. 429 011 IHC; tested species: mouse

Cat. No.: 429 011

Amount: 100 µg

Price: $420.00

ICC
(2 img)

IHC
(2 img)

IHC-P (FFPE)
(5 img)

IHC-Fr
(2 img)

Background

Aquaporin 4 (AQP4) is the most abundant bidirectional water-channel in the central nervous system. It is particularly abundant in astrocytes, where it is highly concentrated in the perivascular end-feet surrounding blood vessels and at the interfaces between brain tissue and cerebrospinal fluid (1). AQP4 is also expressed in ependymal cells and, outside the CNS, in tissues including the kidney, skeletal muscle, lung, stomach and several glandular epithelia (2,3).
It contributes to the regulation of water and ion homeostasis, extracellular volume, brain edema and blood–brain barrier function. AQP4 is also an important component of the glymphatic system, which facilitates the movement and clearance of substances through the brain interstitial space (1,4).
Alterations in AQP4 expression or localization have been implicated in a number of pathological conditions including neuroinflammation, brain edema, traumatic brain injury, neurodegenerative disorders and neuromyelitis optica spectrum disorders (NMOSD)(4).

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Nagelhus EA et al., Physiol. Rev. (2013)
2 ) Palazzo C et al., Int J Mol Sci (2020)
3 ) Gleiser C et al., Int J Mol Sci (2016)
4 ) Wang Y et al., Aging Dis (2022)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

IHC-Fr

Standard Condition

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Aquaporin4 antibody - 429 011

References for Aquaporin4 - 429 011