Synaptic Systems - Aquaporin4

Cat. No.: 429 004

Amount: 100 µl

Price: $375.00

Cat. No. 429 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 5000 up to 1 : 10000 AP staining gallery IP: yes ICC: 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 1000 gallery
Immunogen	Recombinant protein corresponding to AA 249 to 323 from mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity	Reacts with: rat (P47863), mouse (P55088-1). Other species not tested yet.
Specificity	Specific for Aquaporin- 4, detects all described isoforms.
Matching control protein/peptide	429-0P
Data sheet	429_004.pdf

References for Aquaporin4 - 429 004

WB (2)
IP (1)
IHC (9)

Vascular Development of Fetal and Postnatal Neocortex of the Pig, the European Wild Boar Sus scrofa.
Sobierajski E, Czubay K, Beemelmans C, Beemelmans C, Meschkat M, Uhlenkamp D, Meyer G, Wahle P
The Journal of comparative neurology (2024) 53212: e70011. 429 004 WB, IHC; tested species: pig

Evaluation of gliovascular functions of Aqp4 readthrough isoforms.
Mueller SM, White KM, Fass SB, Chen S, Shi Z, Ge X, Engelbach JA, Gaines SH, Bice AR, Vasek MJ, Garbow JR, et al.
bioRxiv : the preprint server for biology (2023) : . 429 004 WB; tested species: mouse

40 Hz light flickering facilitates the glymphatic flow via adenosine signaling in mice.
Sun X, Dias L, Peng C, Zhang Z, Ge H, Wang Z, Jin J, Jia M, Xu T, Guo W, Zheng W, et al.
Cell discovery (2024) 101: 81. 429 004 IP; tested species: mouse

Astrocyte TrkB promotes brain injury and edema formation in ischemic stroke.
Colombo E, Bacigaluppi M, Bartoccetti M, Triolo D, Bassani C, Bergamaschi A, Descamps HC, Gullotta GS, Henley M, Piccoli M, Anastasia L, et al.
Neurobiology of disease (2024) 201: 106670. 429 004 IHC; tested species: mouse

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 429 004 IHC; tested species: human

Microglia directly associate with pericytes in the central nervous system.
Morris GP, Foster CG, Courtney JM, Collins JM, Cashion JM, Brown LS, Howells DW, DeLuca GC, Canty AJ, King AE, Ziebell JM, et al.
Glia (2023) : . 429 004 IHC; tested species: mouse

The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.
Tóth K, Lénárt N, Berki P, Fekete R, Szabadits E, Pósfai B, Cserép C, Alatshan A, Benkő S, Kiss D, Hübner CA, et al.
PLoS biology (2022) 201: e3001526. 429 004 IHC; tested species: mouse

Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions.
Császár E, Lénárt N, Cserép C, Környei Z, Fekete R, Pósfai B, Balázsfi D, Hangya B, Schwarcz AD, Szabadits E, Szöllősi D, et al.
The Journal of experimental medicine (2022) 2193: . 429 004 IHC; tested species: mouse

Update on Perineuronal Net Staining With Wisteria floribunda Agglutinin (WFA).
Härtig W, Meinicke A, Michalski D, Schob S, Jäger C
Frontiers in integrative neuroscience (2022) 16: 851988. 429 004 IHC; tested species: mouse

SARS-CoV-2 infects neurons and induces neuroinflammation in a non-human primate model of COVID-19.
Beckman D, Bonillas A, Diniz GB, Ott S, Roh JW, Elizaldi SR, Schmidt BA, Sammak RL, Van Rompay KKA, Iyer SS, Morrison JH, et al.
Cell reports (2022) 415: 111573. 429 004 IHC; tested species: monkey

Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep.
Michalski D, Reimann W, Spielvogel E, Mages B, Biedermann B, Barthel H, Nitzsche B, Schob S, Härtig W
International journal of molecular sciences (2022) 2311: . 429 004 IHC; tested species: mouse

Cat. No.: 429 004

Amount: 100 µl

Price: $375.00

References
WB (2)
IP (1)
IHC (9)

ICC

IHC

IHC-P

Background

Aquaporin4, or AQP4, also known as Mercurial-insensitive water channel (MIWC), is a member of the aquaporin water channel family. This osmoreceptor regulates body water balance and mediates water flow within the central nervous system.

1 ) Mack AF et al., Brain Struct Funct (2018)
2 ) Amann B et al., Int J Mol Sci (2016)
3 ) Nagelhus EA et al., Physiol. Rev. (2013)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

Aquaporin4 antibody - 429 004

References for Aquaporin4 - 429 004