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Home > Products > 429 004

Aquaporin4 antibody - 429 004

Aquaporin4 is a water-specific channel in the blood brain barrier
Guinea pig polyclonal antiserum
Cat. No.: 429 004
Amount: 100 µl
Price: $370.00
Cat. No. 429 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 5000 up to 1 : 10000 AP staining gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 249 to 323 from mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity Reacts with: rat (P47863), mouse (P55088-1).
Other species not tested yet.
Specificity Specific for Aquaporin- 4, detects all described isoforms.
Matching control protein/peptide 429-0P
Data sheet 429_004.pdf

References for Aquaporin4 - 429 004

Evaluation of gliovascular functions of Aqp4 readthrough isoforms.
Mueller SM, White KM, Fass SB, Chen S, Shi Z, Ge X, Engelbach JA, Gaines SH, Bice AR, Vasek MJ, Garbow JR, et al.
bioRxiv : the preprint server for biology (2023) : . 429 004 WB; tested species: mouse
40 Hz light flickering facilitates the glymphatic flow via adenosine signaling in mice.
Sun X, Dias L, Peng C, Zhang Z, Ge H, Wang Z, Jin J, Jia M, Xu T, Guo W, Zheng W, et al.
Cell discovery (2024) 101: 81. 429 004 IP; tested species: mouse
Astrocyte TrkB promotes brain injury and edema formation in ischemic stroke.
Colombo E, Bacigaluppi M, Bartoccetti M, Triolo D, Bassani C, Bergamaschi A, Descamps HC, Gullotta GS, Henley M, Piccoli M, Anastasia L, et al.
Neurobiology of disease (2024) 201: 106670. 429 004 IHC; tested species: mouse
Microglia directly associate with pericytes in the central nervous system.
Morris GP, Foster CG, Courtney JM, Collins JM, Cashion JM, Brown LS, Howells DW, DeLuca GC, Canty AJ, King AE, Ziebell JM, et al.
Glia (2023) : . 429 004 IHC; tested species: mouse
The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.
Tóth K, Lénárt N, Berki P, Fekete R, Szabadits E, Pósfai B, Cserép C, Alatshan A, Benkő S, Kiss D, Hübner CA, et al.
PLoS biology (2022) 201: e3001526. 429 004 IHC; tested species: mouse
Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions.
Császár E, Lénárt N, Cserép C, Környei Z, Fekete R, Pósfai B, Balázsfi D, Hangya B, Schwarcz AD, Szabadits E, Szöllősi D, et al.
The Journal of experimental medicine (2022) 2193: . 429 004 IHC; tested species: mouse
Update on Perineuronal Net Staining With Wisteria floribunda Agglutinin (WFA).
Härtig W, Meinicke A, Michalski D, Schob S, Jäger C
Frontiers in integrative neuroscience (2022) 16: 851988. 429 004 IHC; tested species: mouse
SARS-CoV-2 infects neurons and induces neuroinflammation in a non-human primate model of COVID-19.
Beckman D, Bonillas A, Diniz GB, Ott S, Roh JW, Elizaldi SR, Schmidt BA, Sammak RL, Van Rompay KKA, Iyer SS, Morrison JH, et al.
Cell reports (2022) 415: 111573. 429 004 IHC; tested species: monkey
Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep.
Michalski D, Reimann W, Spielvogel E, Mages B, Biedermann B, Barthel H, Nitzsche B, Schob S, Härtig W
International journal of molecular sciences (2022) 2311: . 429 004 IHC; tested species: mouse
Cat. No.: 429 004
Amount: 100 µl
Price: $370.00
Evaluation of gliovascular functions of Aqp4 readthrough isoforms.
Mueller SM, White KM, Fass SB, Chen S, Shi Z, Ge X, Engelbach JA, Gaines SH, Bice AR, Vasek MJ, Garbow JR, et al.
bioRxiv : the preprint server for biology (2023) : . 429 004 WB; tested species: mouse
40 Hz light flickering facilitates the glymphatic flow via adenosine signaling in mice.
Sun X, Dias L, Peng C, Zhang Z, Ge H, Wang Z, Jin J, Jia M, Xu T, Guo W, Zheng W, et al.
Cell discovery (2024) 101: 81. 429 004 IP; tested species: mouse
Astrocyte TrkB promotes brain injury and edema formation in ischemic stroke.
Colombo E, Bacigaluppi M, Bartoccetti M, Triolo D, Bassani C, Bergamaschi A, Descamps HC, Gullotta GS, Henley M, Piccoli M, Anastasia L, et al.
Neurobiology of disease (2024) 201: 106670. 429 004 IHC; tested species: mouse
Microglia directly associate with pericytes in the central nervous system.
Morris GP, Foster CG, Courtney JM, Collins JM, Cashion JM, Brown LS, Howells DW, DeLuca GC, Canty AJ, King AE, Ziebell JM, et al.
Glia (2023) : . 429 004 IHC; tested species: mouse
The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.
Tóth K, Lénárt N, Berki P, Fekete R, Szabadits E, Pósfai B, Cserép C, Alatshan A, Benkő S, Kiss D, Hübner CA, et al.
PLoS biology (2022) 201: e3001526. 429 004 IHC; tested species: mouse
Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions.
Császár E, Lénárt N, Cserép C, Környei Z, Fekete R, Pósfai B, Balázsfi D, Hangya B, Schwarcz AD, Szabadits E, Szöllősi D, et al.
The Journal of experimental medicine (2022) 2193: . 429 004 IHC; tested species: mouse
Update on Perineuronal Net Staining With Wisteria floribunda Agglutinin (WFA).
Härtig W, Meinicke A, Michalski D, Schob S, Jäger C
Frontiers in integrative neuroscience (2022) 16: 851988. 429 004 IHC; tested species: mouse
SARS-CoV-2 infects neurons and induces neuroinflammation in a non-human primate model of COVID-19.
Beckman D, Bonillas A, Diniz GB, Ott S, Roh JW, Elizaldi SR, Schmidt BA, Sammak RL, Van Rompay KKA, Iyer SS, Morrison JH, et al.
Cell reports (2022) 415: 111573. 429 004 IHC; tested species: monkey
Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep.
Michalski D, Reimann W, Spielvogel E, Mages B, Biedermann B, Barthel H, Nitzsche B, Schob S, Härtig W
International journal of molecular sciences (2022) 2311: . 429 004 IHC; tested species: mouse
WB
ICC
IHC
IHC-P
Background

Aquaporin4, or AQP4, also known as Mercurial-insensitive water channel (MIWC), is a member of the aquaporin water channel family. This osmoreceptor regulates body water balance and mediates water flow within the central nervous system.
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