Acetylcholinesterase (AChE) antibody - 425 011

AChE terminates the signal transmission at the cholinergic synapses

Mouse monoclonal purified IgG

Cat. No.: 425 011

Amount: 100 µg

Price: $415.00

Cat. No. 425 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	35F8E8
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 489 to 614 from rat AChE (UniProt Id: P37136)
Reactivity	Reacts with: mouse (P21836), rat (P37136). Other species not tested yet.
Specificity	Specific for AChE, may cross react to isoforms R and H.
Remarks	IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended.
Data sheet	425_011.pdf

Cat. No.: 425 011

Amount: 100 µg

Price: $415.00

IHC

IHC-P

Background

Acetylcholinesterase also known as AChE or Acetylhydrolase, hydrolyzes the neurotransmitter acetylcholine at neuromuscular junctions and cholinergic synapses in the brain and thus terminates signal transmission. The protein is also found on the membranes of erythrocytes.

Acetylcholinesterase is a globular, glycosylated protein that lacks a transmembrane peptide-anchor region and occurs as several isoforms, originating from alternative splicing of the AChE mRNA.

1 ) Soreq H et al., Nat. Rev. Neurosci. (2001)
2 ) Hedreen JC et al., J. Histochem. Cytochem. (1985)
3 ) Grisaru D et al., Eur. J. Biochem. (1999)
4 ) Henderson Z et al., Brain Res. (2010)
5 ) Paul R et al., Sci Rep (2017)

Protocols

IHC

IHC-P

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