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Acetylcholinesterase (AChE) - 425 011

Terminates the signal transmission at the cholinergic synapses.
Monoclonal mouse purified IgG
Cat. No.: 425 011
Amount: 100 µg
Price: $415.00
Cat. No. 425 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 1000 gallery  
Clone 35F8E8
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 489 to 614 from rat AChE (UniProt Id: P37136)Recombinant protein corresponding to AA 489 to 614 from rat AChE isoform T (UniProt Id: P37136).
Reactivity Reacts with: mouse (P21836), rat (P37136).
Other species not tested yet.
Specificity Specific for AChE, may cross react to isoforms R and H.
Remarks

IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended.

Data sheet 425_011.pdf
Cat. No.: 425 011
Quantity: 100 µg
Price: $415.00
Background

Acetylcholinesterase also known as AChE or Acetylhydrolase, hydrolyzes the neurotransmitter acetylcholine at neuromuscular junctions and cholinergic synapses in the brain and thus terminates signal transmission. The protein is also found on the membranes of erythrocytes.

Acetylcholinesterase is a globular, glycosylated protein that lacks a transmembrane peptide-anchor region and occurs as several isoforms, originating from alternative splicing of the AChE mRNA.