CPT1c antibody - 415 003 K.O.

CPT1c is a brain specific carnitine palmitoyltransferase 1 isoform

Rabbit polyclonal purified antibody

Cat. No.: 415 003

Amount: 50 µg

Price: $375.00

Cat. No. 415 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 784 to 798 from mouse Cpt1c (UniProt Id: Q8BGD5)
Reactivity	Reacts with: mouse (Q8BGD5), rat (F1LN46). Other species not tested yet.
Specificity	K.O. validated PubMed: 37309891
Matching control protein/peptide	415-0P
Data sheet	415_003.pdf

References for CPT1c - 415 003

CPT1C is required for synaptic plasticity and oscillatory activity that supports motor, associative and non-associative learning.
Iborra-Lázaro G, Djebari S, Sánchez-Rodríguez I, Gratacòs-Batlle E, Sánchez-Fernández N, Radošević M, Casals N, Navarro-López JD, Soto Del Cerro D, Jiménez-Díaz L
The Journal of physiology (2023) : . 415 003 IHC; KO verified; tested species: mouse

Cat. No.: 415 003

Amount: 50 µg

Price: $375.00

ICC

IHC

Background

CPT1c is a carnitine palmitoyltransferase 1 (CPT 1) isoform that is expressed only in the brain. It has been shown to be an integral part of native AMPA receptor complexes in neurons and modulates the surface expression of these ionotropic glutamate receptors.

1 ) Gratacòs-Batlle E et al., Front Mol Neurosci (2018)
2 ) Wang R et al., J Cancer (2017)
3 ) Fadó R et al., J. Biol. Chem. (2015)
4 ) Gratacòs-Batlle E et al., Front Cell Neurosci (2014)
5 ) Sierra AY et al., J. Biol. Chem. (2008)
6 ) Dai Y et al., Biochem. Biophys. Res. Commun. (2007)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

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