Synaptic Systems

Cat. No.: 411 004

Amount: 100 µl

Price: $370.00

Cat. No. 411 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 2000 up to 1 : 10000 (see remarks) gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of mouse Brn3a (UniProt Id: P17208)
Reactivity	Reacts with: mouse (P17208). Other species not tested yet.
Remarks	IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.
Data sheet	411_004.pdf

References for Brn3a - 411 004

Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projections.
Fries M, Brown TW, Jolicoeur C, Boulan B, Boudreau-Pinsonneault C, Javed A, Abram P, Cayouette M
Cell reports (2023) 428: 112985. 411 004 IHC; tested species: mouse

Methods to Identify Rat and Mouse Retinal Ganglion Cells in Retinal Flat-Mounts.
Miralles de Imperial-Ollero JA, Vidal-Villegas B, Gallego-Ortega A, Nadal-Nicolás FM, Salinas-Navarro M, Norte-Muñoz M, Di Pierdomenico J, Galindo-Romero C, Agudo-Barriuso M, Vidal-Sanz M, Valiente-Soriano FJ, et al.
Methods in molecular biology (Clifton, N.J.) (2023) 2708: 175-194. 411 004 IHC; tested species: mouse

Diversity in homeostatic calcium set points predicts retinal ganglion cell survival following optic nerve injury in vivo.
McCracken S, Fitzpatrick MJ, Hall AL, Wang Z, Kerschensteiner D, Morgan JL, Williams PR
Cell reports (2023) 4210: 113165. 411 004 IHC; tested species: mouse

Alpha retinal ganglion cells in pigmented mice retina: number and distribution.
Gallego-Ortega A, Norte-Muñoz M, Di Pierdomenico J, Avilés-Trigueros M, de la Villa P, Valiente-Soriano FJ, Vidal-Sanz M
Frontiers in neuroanatomy (2022) 16: 1054849. 411 004 IHC; tested species: mouse

Cat. No.: 411 004

Amount: 100 µl

Price: $370.00

IHC

IHC-P

Background

Brn3a, also referred to as POU4F1, RGC-1 or Oct-T1, is a transcription factor highly expressed in the developing peripheral sensory nervous system, in cells of the B- and T-lymphocytic lineages and in certain regions of the CNS e.g. retina, spinal cord, midbrain superior colliculus, red nucleus, nucleus ambiguus, inferior olivary nucleus and habenula. In the retina Brn3a is a well-established marker for retinal ganglion cells.

1 ) Ghinia MG et al., J. Comp. Neurol. (2016)
2 ) Dykes IM et al., J. Neurosci. (2011)
3 ) Smith MD et al., Brain Res. Mol. Brain Res. (1999)
4 ) Xiang M et al., Proc. Natl. Acad. Sci. U.S.A. (1996)
5 ) Budhram-Mahadeo V et al., J. Biol. Chem. (1996)
6 ) Xiang M et al., J. Neurosci. (1995)
7 ) Lillycrop KA et al., Nucleic Acids Res. (1992)

Protocols

IHC

IHC-P

Other products for this target

Brn3a antibody - 411 004

References for Brn3a - 411 004