Synaptic Systems

Cat. No.: 409 006

Amount: 50 µg

Price: $385.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 409 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 1:500 up to 1 : 1:1000 gallery IHC: 1 : 1:500 up to 1 : 1:1000 gallery IHC-P: 1 : 1:500 up to 1 : 1:1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 219 from sea anemone mScarlet
Specificity	Recognizes mScarlet, mRFP, mCherry and tdTomato.
Data sheet	409_006.pdf

References for RFP - 409 006

Senescence-induced cellular reprogramming drives cnidarian whole-body regeneration.
Salinas-Saavedra M, Febrimarsa , Krasovec G, Horkan HR, Baxevanis AD, Frank U
Cell reports (2023) 427: 112687. 409 006 ICC

Endocytosis in the axon initial segment maintains neuronal polarity.
Eichel K, Uenaka T, Belapurkar V, Lu R, Cheng S, Pak JS, Taylor CA, Südhof TC, Malenka R, Wernig M, Özkan E, et al.
Nature (2022) : . 409 006 ICC; tested species: rat

The vSNAREs VAMP2 and VAMP4 control recycling and intracellular sorting of post-synaptic receptors in neuronal dendrites.
Bakr M, Jullié D, Krapivkina J, Paget-Blanc V, Bouit L, Petersen JD, Retailleau N, Breillat C, Herzog E, Choquet D, Perrais D, et al.
Cell reports (2021) 3610: 109678. 409 006 ICC

Cat. No.: 409 006

Amount: 50 µg

Price: $385.00

ICC

IHC

IHC-P

Background

Red fluorescent protein RFP and its derivates have become universal tools in cell biology. Most RFPs derive from a protein isolated from Discosoma sp. They are used as fluorescent tags to investigate expression levels, patterns and protein localization.

1 ) Fink D et al., Genesis (2010)
2 ) Shaner NC et al., Nat. Biotechnol. (2004)
3 ) Labas YA et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
4 ) Fradkov AF et al., FEBS Lett. (2000)

Protocols

ICC

IHC

IHC-P

Other products for this target

RFP antibody - 409 006

References for RFP - 409 006