Synaptic Systems

Cat. No.: 400 211

Amount: 100 µg

Price: $415.00

Cat. No. 400 211	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	97G1C1
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 189 to 283 from rat TMEM119 (UniProt Id: B2RYL3)
Reactivity	Reacts with: rat (B2RYL3). Other species not tested yet.
Remarks	This antibody is recommended for rat only. Due to significant differences of TMEM 119 among species, cross-reactivity is unlikely.
Data sheet	400_211.pdf

References for TMEM119 - 400 211

Neurosteroid [3α,5α]-3-Hydroxy-pregnan-20-one Enhances the CX3CL1-CX3CR1 Pathway in the Brain of Alcohol-Preferring Rats with Sex-Specificity.
Balan I, Grusca A, Chéry SL, Materia BR, O'Buckley TK, Morrow AL
Life (Basel, Switzerland) (2024) 147: . 400 211 IHC; tested species: rat

Radiotherapy induces persistent innate immune reprogramming of microglia into a primed state.
Voshart DC, Oshima T, Jiang Y, van der Linden GP, Ainslie AP, Reali Nazario L, van Buuren-Broek F, Scholma AC, van Weering HRJ, Brouwer N, Sewdihal J, et al.
Cell reports (2024) 432: 113764. 400 211 IHC; tested species: rat

Integrated inflammatory signaling landscape response after delivering Elovanoid free-fatty-acid precursors leading to experimental stroke neuroprotection.
Reid MM, Belayev L, Khoutorova L, Mukherjee PK, Obenaus A, Shelvin K, Knowles S, Hong SH, Bazan NG
Scientific reports (2023) 131: 15841. 400 211 IHC; tested species: rat

Destructive Effect of Intravitreal Heat Shock Protein 27 Application on Retinal Ganglion Cells and Neurofilament.
Grotegut P, Kuehn S, Dick HB, Joachim SC
International journal of molecular sciences (2020) 212: . 400 211 IHC; tested species: rat

Cat. No.: 400 211

Amount: 100 µg

Price: $415.00

IHC

IHC-P

Background

Microglia are resident myeloid cells of the central nervous system (CNS). They are ontogenetically and functionally distinct from monocyte-derived macrophages that infiltrate the CNS under pathological conditions. Transmembrane protein 119 (TMEM119) is a single-pass type I membrane protein that has been identified as a useful, highly selective microglia marker protein.

1 ) Bennett ML et al., Proc. Natl. Acad. Sci. U.S.A. (2016)
2 ) Satoh J et al., Neuropathology (2016)

Protocols

IHC

IHC-P

Other products for this target

TMEM119 antibody rat specific - 400 211

References for TMEM119 - 400 211