TMEM119 antibody human specific - 400 102

TMEM119 is a microglia specific transmembrane protein

Rabbit polyclonal antiserum

Cat. No.: 400 102

Amount: 200 µl

Price: $355.00

Cat. No. 400 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 194 to 283 from human TMEM119 (UniProt Id: Q4V9L6)
Reactivity	Reacts with: human (Q4V9L6). Other species not tested yet.
Remarks	This antibody is recommended for material from human only. Due to significant differences of TMEM 119 among species, cross-reactivity is unlikely.
Data sheet	400_102.pdf

References for TMEM119 - 400 102

A modified protocol for isolation of retinal microglia from the pig.
Zhang Y, Erhard AL, Plagemann T, Eter N, Heiduschka P
Experimental eye research (2021) 207: 108584. 400 102 ICC; tested species: pig

Distinct tau neuropathology and cellular profiles of an APOE3 Christchurch homozygote protected against autosomal dominant Alzheimer's dementia.
Sepulveda-Falla D, Sanchez JS, Almeida MC, Boassa D, Acosta-Uribe J, Vila-Castelar C, Ramirez-Gomez L, Baena A, Aguillon D, Villalba-Moreno ND, Littau JL, et al.
Acta neuropathologica (2022) 1443: 589-601. 400 102 IHC; tested species: human

Cat. No.: 400 102

Amount: 200 µl

Price: $355.00

IHC-P

Background

Microglia are resident myeloid cells of the central nervous system (CNS). They are ontogenetically and functionally distinct from monocyte-derived macrophages that infiltrate the CNS under pathological conditions. Transmembrane protein 119 (TMEM119) is a single-pass type I membrane protein that has been identified as a useful, highly selective microglia marker protein.

1 ) Bennett ML et al., Proc. Natl. Acad. Sci. U.S.A. (2016)
2 ) Satoh J et al., Neuropathology (2016)

Protocols

IHC-P

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