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Home > Products > 400 011

TMEM119 antibody mouse specific - 400 011

TMEM119 is a microglia specific transmembrane protein
Mouse monoclonal purified IgG
Cat. No.: 400 011
Amount: 100 µg
Price: $430.00
Cat. No. 400 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 100 up to 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 195H4
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to the C-terminal region of mouse TMEM119 (UniProt Id: Q8R138)
Reactivity Reacts with: mouse (Q8R138).
No signal: rat (B2RYL3), human (Q4V9L6).
Other species not tested yet.
Remarks

This antibody is recommended for mouse only. Due to significant differences of TMEM 119 among species, cross-reactivity is unlikely.
Data sheet 400_011.pdf

References for TMEM119 - 400 011

Bacterial peptidoglycan signalling in microglia: Activation by MDP via the NF-κB/MAPK pathway.
Spielbauer J, Glotfelty E, Sarlus H, Harris RA, Heijtz RD, Karlsson TE
Brain, behavior, and immunity (2024) : . 400 011 ICC; tested species: mouse
The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.
Glotfelty EJ, Tovar-Y-Romo LB, Hsueh SC, Tweedie D, Li Y, Harvey BK, Hoffer BJ, Karlsson TE, Olson L, Greig NH
Cells (2023) 1210: . 400 011 ICC, IHC; tested species: mouse
Pharmacological Upregulation of Microglial Lipid Droplet Alleviates Neuroinflammation and Acute Ischemic Brain Injury.
Li H, Liu P, Deng S, Zhu L, Cao X, Bao X, Xia S, Xu Y, Zhang B
Inflammation (2023) : . 400 011 ICC, IHC; tested species: mouse
Loss of Epitranscriptomic Modification N6-Methyladenosine (m6A) Reader YTHDF1 Exacerbates Ischemic Brain Injury in a Sexually Dimorphic Manner.
Chokkalla AK, Arruri V, Mehta SL, Vemuganti R
Translational stroke research (2024) : . 400 011 IHC; tested species: mouse
CD11c+ microglia promote white matter repair after ischemic stroke.
Jia J, Zheng L, Ye L, Chen J, Shu S, Xu S, Bao X, Xia S, Liu R, Xu Y, Zhang M, et al.
Cell death & disease (2023) 142: 156. 400 011 IHC; tested species: mouse
The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.
Glotfelty EJ, Tovar-Y-Romo LB, Hsueh SC, Tweedie D, Li Y, Harvey BK, Hoffer BJ, Karlsson TE, Olson L, Greig NH
Cells (2023) 1210: . 400 011 ICC, IHC; tested species: mouse
Pharmacological Upregulation of Microglial Lipid Droplet Alleviates Neuroinflammation and Acute Ischemic Brain Injury.
Li H, Liu P, Deng S, Zhu L, Cao X, Bao X, Xia S, Xu Y, Zhang B
Inflammation (2023) : . 400 011 ICC, IHC; tested species: mouse
Acute ischemia induces spatially and transcriptionally distinct microglial subclusters.
Li H, Liu P, Zhang B, Yuan Z, Guo M, Zou X, Qian Y, Deng S, Zhu L, Cao X, Tao T, et al.
Genome medicine (2023) 151: 109. 400 011 IHC; tested species: mouse
Posttranslational S-nitrosylation modification regulates HMGB1 secretion and promotes its proinflammatory and neurodegenerative effects.
Yang R, Gao Y, Li H, Huang W, Tu D, Yang M, Liu X, Hong JS, Gao HM
Cell reports (2022) 4011: 111330. 400 011 IHC; tested species: mouse
Haploinsufficiency of microglial MyD88 ameliorates Alzheimer's pathology and vascular disorders in APP/PS1-transgenic mice.
Quan W, Luo Q, Hao W, Tomic I, Furihata T, Schulz-Schäffer W, Menger MD, Fassbender K, Liu Y
Glia (2021) : . 400 011 IHC; tested species: mouse
Prolonged NCX activation prevents SOD1 accumulation, reduces neuroinflammation, ameliorates motor behavior and prolongs survival in a ALS mouse model.
Anzilotti S, Valsecchi V, Brancaccio P, Guida N, Laudati G, Tedeschi V, Petrozziello T, Frecentese F, Magli E, Hassler B, Cuomo O, et al.
Neurobiology of disease (2021) 159: 105480. 400 011 IHC; tested species: mouse
Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction.
Pan J, Ma N, Zhong J, Yu B, Wan J, Zhang W
Molecular therapy. Nucleic acids (2021) 26: 970-986. 400 011 IHC; tested species: mouse
Deletion of ubiquitin ligase Nedd4l exacerbates ischemic brain damage.
Kim T, Chokkalla AK, Vemuganti R
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2020) : 271678X20943804. 400 011 IHC; tested species: mouse
Dual microglia effects on blood brain barrier permeability induced by systemic inflammation.
Haruwaka K, Ikegami A, Tachibana Y, Ohno N, Konishi H, Hashimoto A, Matsumoto M, Kato D, Ono R, Kiyama H, Moorhouse AJ, et al.
Nature communications (2019) 101: 5816. 400 011 IHC; tested species: mouse
Tenascin-C induction exacerbates post-stroke brain damage.
Chelluboina B, Chokkalla AK, Mehta SL, Morris-Blanco KC, Bathula S, Sankar S, Park JS, Vemuganti R
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2021) : 271678X211056392. 400 011 IHC-P; tested species: mouse
Cat. No.: 400 011
Amount: 100 µg
Price: $430.00
Bacterial peptidoglycan signalling in microglia: Activation by MDP via the NF-κB/MAPK pathway.
Spielbauer J, Glotfelty E, Sarlus H, Harris RA, Heijtz RD, Karlsson TE
Brain, behavior, and immunity (2024) : . 400 011 ICC; tested species: mouse
The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.
Glotfelty EJ, Tovar-Y-Romo LB, Hsueh SC, Tweedie D, Li Y, Harvey BK, Hoffer BJ, Karlsson TE, Olson L, Greig NH
Cells (2023) 1210: . 400 011 ICC, IHC; tested species: mouse
Pharmacological Upregulation of Microglial Lipid Droplet Alleviates Neuroinflammation and Acute Ischemic Brain Injury.
Li H, Liu P, Deng S, Zhu L, Cao X, Bao X, Xia S, Xu Y, Zhang B
Inflammation (2023) : . 400 011 ICC, IHC; tested species: mouse
Loss of Epitranscriptomic Modification N6-Methyladenosine (m6A) Reader YTHDF1 Exacerbates Ischemic Brain Injury in a Sexually Dimorphic Manner.
Chokkalla AK, Arruri V, Mehta SL, Vemuganti R
Translational stroke research (2024) : . 400 011 IHC; tested species: mouse
CD11c+ microglia promote white matter repair after ischemic stroke.
Jia J, Zheng L, Ye L, Chen J, Shu S, Xu S, Bao X, Xia S, Liu R, Xu Y, Zhang M, et al.
Cell death & disease (2023) 142: 156. 400 011 IHC; tested species: mouse
The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.
Glotfelty EJ, Tovar-Y-Romo LB, Hsueh SC, Tweedie D, Li Y, Harvey BK, Hoffer BJ, Karlsson TE, Olson L, Greig NH
Cells (2023) 1210: . 400 011 ICC, IHC; tested species: mouse
Pharmacological Upregulation of Microglial Lipid Droplet Alleviates Neuroinflammation and Acute Ischemic Brain Injury.
Li H, Liu P, Deng S, Zhu L, Cao X, Bao X, Xia S, Xu Y, Zhang B
Inflammation (2023) : . 400 011 ICC, IHC; tested species: mouse
Acute ischemia induces spatially and transcriptionally distinct microglial subclusters.
Li H, Liu P, Zhang B, Yuan Z, Guo M, Zou X, Qian Y, Deng S, Zhu L, Cao X, Tao T, et al.
Genome medicine (2023) 151: 109. 400 011 IHC; tested species: mouse
Posttranslational S-nitrosylation modification regulates HMGB1 secretion and promotes its proinflammatory and neurodegenerative effects.
Yang R, Gao Y, Li H, Huang W, Tu D, Yang M, Liu X, Hong JS, Gao HM
Cell reports (2022) 4011: 111330. 400 011 IHC; tested species: mouse
Haploinsufficiency of microglial MyD88 ameliorates Alzheimer's pathology and vascular disorders in APP/PS1-transgenic mice.
Quan W, Luo Q, Hao W, Tomic I, Furihata T, Schulz-Schäffer W, Menger MD, Fassbender K, Liu Y
Glia (2021) : . 400 011 IHC; tested species: mouse
Prolonged NCX activation prevents SOD1 accumulation, reduces neuroinflammation, ameliorates motor behavior and prolongs survival in a ALS mouse model.
Anzilotti S, Valsecchi V, Brancaccio P, Guida N, Laudati G, Tedeschi V, Petrozziello T, Frecentese F, Magli E, Hassler B, Cuomo O, et al.
Neurobiology of disease (2021) 159: 105480. 400 011 IHC; tested species: mouse
Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction.
Pan J, Ma N, Zhong J, Yu B, Wan J, Zhang W
Molecular therapy. Nucleic acids (2021) 26: 970-986. 400 011 IHC; tested species: mouse
Deletion of ubiquitin ligase Nedd4l exacerbates ischemic brain damage.
Kim T, Chokkalla AK, Vemuganti R
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2020) : 271678X20943804. 400 011 IHC; tested species: mouse
Dual microglia effects on blood brain barrier permeability induced by systemic inflammation.
Haruwaka K, Ikegami A, Tachibana Y, Ohno N, Konishi H, Hashimoto A, Matsumoto M, Kato D, Ono R, Kiyama H, Moorhouse AJ, et al.
Nature communications (2019) 101: 5816. 400 011 IHC; tested species: mouse
Tenascin-C induction exacerbates post-stroke brain damage.
Chelluboina B, Chokkalla AK, Mehta SL, Morris-Blanco KC, Bathula S, Sankar S, Park JS, Vemuganti R
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2021) : 271678X211056392. 400 011 IHC-P; tested species: mouse
IHC
IHC
IHC-P
Background

Microglia are resident myeloid cells of the central nervous system (CNS). They are ontogenetically and functionally distinct from monocyte-derived macrophages that infiltrate the CNS under pathological conditions. Transmembrane protein 119 (TMEM119) is a single-pass type I membrane protein that has been identified as a useful, highly selective microglia marker protein.
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