Synaptic Systems

Cat. No.: 400 004

Amount: 100 µl

Price: $370.00

Cat. No. 400 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery IHC-Fr: 1 : 100 (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to the C-terminal region of mouse TMEM119 (UniProt Id: Q8R138)
Reactivity	Reacts with: mouse (Q8R138). Other species not tested yet.
Specificity	K.D. validated PubMed: 37635351
Remarks	This antibody is recommended for mouse only. Due to significant differences of TMEM 119 among species, cross-reactivity is unlikely. IHC-Fr: 5 min MeOH and PFA fixation are possible.
Data sheet	400_004.pdf

References for TMEM119 - 400 004

Transcriptome and proteome profiling reveals TREM2-dependent and -independent glial response and metabolic perturbation in an Alzheimer's mouse model.
Lin D, Kaye S, Chen M, Lyanna A, Ye L, Hammond LA, Gao J
The Journal of biological chemistry (2024) : 107874. 400 004 IHC; tested species: mouse

Repopulated microglia after pharmacological depletion decrease dendritic spine density in adult mouse brain.
Wickel J, Chung HY, Ceanga M, von Stackelberg N, Hahn N, Candemir Ö, Baade-Büttner C, Mein N, Tomasini P, Woldeyesus DM, Andreas N, et al.
Glia (2024) : . 400 004 IHC; tested species: mouse

Human-induced pluripotent stem cell-derived microglia integrate into mouse retina and recapitulate features of endogenous microglia.
Ma W, Zhao L, Xu B, Fariss RN, Redmond TM, Zou J, Wong WT, Li W
eLife (2024) 12: . 400 004 IHC; tested species: mouse

CCR3 plays a role in murine age-related cognitive changes and T-cell infiltration into the brain.
Rege SV, Teichert A, Masumi J, Dhande OS, Harish R, Higgins BW, Lopez Y, Akrapongpisak L, Hackbart H, Caryotakis S, Leone DP, et al.
Communications biology (2023) 61: 292. 400 004 IHC; tested species: mouse

A comparative analysis of microglial inducible Cre lines.
Faust TE, Feinberg PA, O'Connor C, Kawaguchi R, Chan A, Strasburger H, Frosch M, Boyle MA, Masuda T, Amann L, Knobeloch KP, et al.
Cell reports (2023) 429: 113031. 400 004 IHC; KD verified; tested species: mouse

URMC-099 prophylaxis prevents hippocampal vascular vulnerability and synaptic damage in an orthopedic model of delirium superimposed on dementia.
Miller-Rhodes P, Li H, Velagapudi R, Chiang W, Terrando N, Gelbard HA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2022) 366: e22343. 400 004 IHC; tested species: mouse

Myeloid Cells in the Mouse Retina and Uveal Tract Respond Differently to Systemic Inflammatory Stimuli.
Dando SJ, Kazanis R, McMenamin PG
Investigative ophthalmology & visual science (2021) 6210: 10. 400 004 IHC; tested species: mouse

The Mononuclear Phagocyte System of the Rat.
Hume DA, Caruso M, Keshvari S, Patkar OL, Sehgal A, Bush SJ, Summers KM, Pridans C, Irvine KM
Journal of immunology (Baltimore, Md. : 1950) (2021) 20610: 2251-2263. 400 004 IHC; tested species: rat

CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10.
Guldner IH, Wang Q, Yang L, Golomb SM, Zhao Z, Lopez JA, Brunory A, Howe EN, Zhang Y, Palakurthi B, Barron M, et al.
Cell (2020) : . 400 004 IHC-P; tested species: mouse

Protecting RNA quality for spatial transcriptomics while improving immunofluorescent staining quality.
Hahn N, Bens M, Kempfer M, Reißig C, Schmidl L, Geis C
Frontiers in neuroscience (2023) 17: 1198154. 400 004 IHC-Fr; tested species: mouse

Cat. No.: 400 004

Amount: 100 µl

Price: $370.00

IHC

IHC-P

Background

Microglia are resident myeloid cells of the central nervous system (CNS). They are ontogenetically and functionally distinct from monocyte-derived macrophages that infiltrate the CNS under pathological conditions. Transmembrane protein 119 (TMEM119) is a single-pass type I membrane protein that has been identified as a useful, highly selective microglia marker protein.

1 ) Bennett ML et al., Proc. Natl. Acad. Sci. U.S.A. (2016)
2 ) Satoh J et al., Neuropathology (2016)

Protocols

IHC

IHC-P

IHC-Fr

Other products for this target

TMEM119 antibody mouse specific - 400 004 K.D.

References for TMEM119 - 400 004