Synaptic Systems

Cat. No.: 398 003

Amount: 200 µl

Price: $455.00

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Detection of proliferation antigen Ki-67 in cells of human (red) and murine (brown) origin in various xenograft models

Integrin-targeting cyclic peptides suppress TGF-β1-driven EMT and invasion in third-generation EGFR-TKI-resistant NSCLC.
Seo J, Kim J
Chemico-biological interactions (2026) 434: 112117. 398 003 IHC; tested species: human

IHC-P (FFPE)
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Background

Ki-67 is a nuclear protein which is widely used as a marker of cell proliferation. Its expression is closely associated with the cell cycle: Ki-67 is present in actively cycling cells during the G1, S, G2, and M phases but is generally absent in quiescent cells in the G0 phase. At the cellular level, Ki-67 is predominantly localized in the nucleus during interphase and redistributes to the periphery of condensed chromosomes during mitosis (1,2).
Immunohistochemical staining for Ki-67 is a simple and reproducible method to determine the tumor proliferative index and is a predictive and prognostic biomarker in certain types of human cancer, such as breast cancer, gastric cancer or prostate cancer. In breast cancer higher Ki-67 levels have been associated with an increased risk of disease recurrence (1,3). However, Ki-67 expression is not specific to malignant cells, as it is also present in normally proliferating tissues and activated lymphocytes.

1 ) Scholzen T et al., J. Cell. Physiol. (2000)
2 ) Sun X et al., Chromosoma (2018)
3 ) de Azambuja E et al., Br J Cancer (2007)

Protocols

IHC-P (FFPE)

Other products for this target

Cat. No. 398 003	200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1251 to 1271 from human Ki67 (UniProt Id: P46013)
Reactivity	Reacts with: human (P46013). No signal: mouse (E9PVX6), pig. Other species not tested yet.
Data sheet	Data Sheet 398_003 Safety Data Sheet (SDS)

Ki67 antibody human specific - 398 003 (HS-398 003)

References for Ki67 - 398 003