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Sorting Nexin4 antibody - 392 003 K.D.

SNX4 is a member of the sorting nexin family
Rabbit polyclonal purified antibody
Cat. No.: 392 003
Amount: 50 µg
Price: $375.00
Cat. No. 392 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 1 to 21 from mouse SNX4 (UniProt Id: Q91YJ2)
Reactivity Reacts with: rat (E9PU13), mouse (Q91YJ2).
Other species not tested yet.
Specificity K.D. validated PubMed: 33106523
Matching control protein/peptide 392-0P
Data sheet 392_003.pdf

References for Sorting Nexin4 - 392 003

The endosomal protein sorting nexin 4 is a synaptic protein.
Vazquez-Sanchez S, Gonzalez-Lozano MA, Walfenzao A, Li KW, van Weering JRT
Scientific reports (2020) 101: 18239. 392 003 WB, ICC, EM; KD verified; tested species: mouse
The endosomal protein sorting nexin 4 is a synaptic protein.
Vazquez-Sanchez S, Gonzalez-Lozano MA, Walfenzao A, Li KW, van Weering JRT
Scientific reports (2020) 101: 18239. 392 003 WB, ICC, EM; KD verified; tested species: mouse
The endosomal protein sorting nexin 4 is a synaptic protein.
Vazquez-Sanchez S, Gonzalez-Lozano MA, Walfenzao A, Li KW, van Weering JRT
Scientific reports (2020) 101: 18239. 392 003 WB, ICC, EM; KD verified; tested species: mouse
Cat. No.: 392 003
Amount: 50 µg
Price: $375.00
The endosomal protein sorting nexin 4 is a synaptic protein.
Vazquez-Sanchez S, Gonzalez-Lozano MA, Walfenzao A, Li KW, van Weering JRT
Scientific reports (2020) 101: 18239. 392 003 WB, ICC, EM; KD verified; tested species: mouse
The endosomal protein sorting nexin 4 is a synaptic protein.
Vazquez-Sanchez S, Gonzalez-Lozano MA, Walfenzao A, Li KW, van Weering JRT
Scientific reports (2020) 101: 18239. 392 003 WB, ICC, EM; KD verified; tested species: mouse
The endosomal protein sorting nexin 4 is a synaptic protein.
Vazquez-Sanchez S, Gonzalez-Lozano MA, Walfenzao A, Li KW, van Weering JRT
Scientific reports (2020) 101: 18239. 392 003 WB, ICC, EM; KD verified; tested species: mouse
Background
Sorting Nexin 4 (SNX 4) is involved in preventing the degradation of endocytosed transferrin receptors and their sorting into the Rab 11a recycling pathway. It may also form complexes with clathrin and dynein and binds several additional receptors for the epidermal growth factor, insulin and leptin.