Synaptic Systems

Cat. No.: 391 005

Amount: 50 µg

Price: $470.00

Cat. No. 391 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 5000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to C-terminal residues of mouse LAMP3. (UniProt Id: Q7TST5)
Reactivity	Reacts with: mouse (Q7TST5), rat (Q5XI99). No signal: human (Q9UQV4). Other species not tested yet.
Specificity	Recognizes LAMP 3, no crossreactivity to LAMP 1, LAMP 2 and LAMP 5. K.O. validated
Data sheet	391_005.pdf

References for LAMP3 - 391 005

Emergence of inflammatory fibroblasts with aging in Hermansky-Pudlak syndrome associated pulmonary fibrosis.
Banaschewski BJH, Michki SN, Sitaraman S, Pan R, Wang JY, Stewart D, Goldy MK, Lin SM, Cantu E, Katzen JB, Basil MC, et al.
Communications biology (2025) 81: 284. 391 005 IHC; tested species: mouse

Stem cell migration drives lung repair in living mice.
Chioccioli M, Liu S, Magruder S, Tata A, Borriello L, McDonough JE, Konkimalla A, Kim SH, Nouws J, Gonzalez DG, Traub B, et al.
Developmental cell (2024) : . 391 005 IHC; tested species: mouse

Dysregulated alveolar epithelial cell progenitor function and identity in Hermansky-Pudlak syndrome.
Wang JY, Michki SN, Sitaraman S, Banaschewski BJ, Jamal R, Gokey JJ, Lin SM, Katzen JB, Basil MC, Cantu E, Kropski JA, et al.
JCI insight (2024) 103: . 391 005 IHC; tested species: mouse

Efficient Adeno-associated Virus-mediated Transgenesis in Alveolar Stem Cells and Associated Niches.
Konkimalla A, Elmore Z, Konishi S, Macadlo L, Katsura H, Tata A, Asokan A, Tata PR
American journal of respiratory cell and molecular biology (2023) 693: 255-265. 391 005 IHC; tested species: mouse

Transitional cell states sculpt tissue topology during lung regeneration.
Konkimalla A, Konishi S, Macadlo L, Kobayashi Y, Farino ZJ, Miyashita N, El Haddad L, Morowitz J, Barkauskas CE, Agarwal P, Souma T, et al.
Cell stem cell (2023) 3011: 1486-1502.e9. 391 005 IHC; tested species: mouse

Multi-apical polarity of alveolar stem cells and their dynamics during lung development and regeneration.
Konkimalla A, Konishi S, Kobayashi Y, Kadur Lakshminarasimha Murthy P, Macadlo L, Mukherjee A, Elmore Z, Kim SJ, Pendergast AM, Lee PJ, Asokan A, et al.
iScience (2022) 2510: 105114. 391 005 IHC; tested species: mouse

Three-axis classification of mouse lung mesenchymal cells reveals two populations of myofibroblasts.
Narvaez Del Pilar O, Gacha Garay MJ, Chen J
Development (Cambridge, England) (2022) 1496: . 391 005 IHC; tested species: mouse

Differential chromatin binding of the lung lineage transcription factor NKX2-1 resolves opposing murine alveolar cell fates in vivo.
Little DR, Lynch AM, Yan Y, Akiyama H, Kimura S, Chen J
Nature communications (2021) 121: 2509. 391 005 IHC; tested species: mouse

Multi-lineage Lung Regeneration by Stem Cell Transplantation across Major Genetic Barriers.
Hillel-Karniel C, Rosen C, Milman-Krentsis I, Orgad R, Bachar-Lustig E, Shezen E, Reisner Y
Cell reports (2020) 303: 807-819.e4. 391 005 IHC; tested species: mouse

PRDM3/16 Regulate Chromatin Accessibility Required for NKX2-1 Mediated Alveolar Epithelial Differentiation and Function.
He H, Bell SM, Davis AK, Zhao S, Sridharan A, Na CL, Guo M, Xu Y, Snowball J, Swarr DT, Zacharias WJ, et al.
bioRxiv : the preprint server for biology (2023) : . 391 005 IHC-P; tested species: mouse

Cat. No.: 391 005

Amount: 50 µg

Price: $470.00

IHC

IHC-P

Background

LAMP 3 (lysosome-associated membrane glycoprotein 3), also referred to as DC-LAMP (dendritic cell LAMP) or CD 208, is a member of the LAMP protein family and a single-pass type I transmembrane protein that can be variably glycosylated. It is specifically expressed in lung type II pneumocytes and by activated human dendritic cells (1, 2).
Recent studies demonstrated high expression of LAMP 3 in a variety of malignancies including squamous cell carcinoma, gastrointestinal cancer, breast cancer, ovarian cancer, and cervical cancer. Its expression has been associated with metastasis and poor overall survival (3, 4, 5).

1 ) de Saint-Vis B et al., Immunity (1998)
2 ) Salaun B et al., Am J Pathol (2004)
3 ) Ozaki K et al., Cancer Res (1998)
4 ) Liao X et al., Int J Mol Sci (2015)
5 ) Wang D et al., Tumour Biol. (2017)

Protocols

IHC

IHC-P

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LAMP3 antibody - 391 005 K.O.

References for LAMP3 - 391 005