Ankyrin G antibody - 386 006

AnkyrinG is a membrane-cytoskeleton linker

Chicken polyclonal purified antibody

Cat. No.: 386 006

Amount: 50 µg

Price: $385.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 386 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 up to 1 : 2000 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Ankyrin G. (UniProt Id: G5E8K5-1)
Reactivity	Reacts with: rat (O70511-1), mouse (G5E8K5-1). Other species not tested yet.
Specificity	Specific for Ankyrin G; detects all described splice variants.
Remarks	IHC: Antigen retrieval (10mM Tris, 1mM EDTA, pH 9.0, overnight at 60°C) is recommended. IHC-Fr: Fixation with acetone or PFA/formaldehyde is recommended. Postfixation with acetone-methanol or methanol is not advised.
Data sheet	386_006.pdf

References for Ankyrin G - 386 006

Immunoproximity biotinylation reveals the axon initial segment proteome.
Zhang W, Fu Y, Peng L, Ogawa Y, Ding X, Rasband A, Zhou X, Shelly M, Rasband MN, Zou P
Nature communications (2023) 141: 8201. 386 006 ICC; tested species: rat

Endocytosis in the axon initial segment maintains neuronal polarity.
Eichel K, Uenaka T, Belapurkar V, Lu R, Cheng S, Pak JS, Taylor CA, Südhof TC, Malenka R, Wernig M, Özkan E, et al.
Nature (2022) : . 386 006 ICC; tested species: mouse

Cat. No.: 386 006

Amount: 50 µg

Price: $385.00

ICC

IHC

IHC-Fr

Background

Ankyrin G is a membrane-cytoskeleton linker. It may participate in the targeting and clustering of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (AIS).

1 ) Fujiwara Y et al., Sci Rep (2016)
2 ) Yang Y et al., J. Cell Biol. (2007)
3 ) Zhou D et al., J. Cell Biol. (1998)
4 ) Wood SJ et al., J. Cell Biol. (1998)
5 ) Kordeli E et al., J. Biol. Chem. (1995)

Protocols

ICC

IHC

IHC-Fr

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