Synaptic Systems - Ankyrin G

Cat. No.: 386 004

Amount: 100 µl

Price: $375.00

Cat. No. 386 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Ankyrin G. (UniProt Id: G5E8K5-1)
Reactivity	Reacts with: rat (O70511-1), mouse (G5E8K5-1). Other species not tested yet.
Specificity	Specific for Ankyrin G; detects all described splice variants. K.D. validated PubMed: 31727776
Data sheet	386_004.pdf

References for Ankyrin G - 386 004

WB (3)
ICC (8)
IHC (7)
IHC-Fr (1)

Neurocan regulates axon initial segment organization and neuronal activity.
Baidoe-Ansah D, Mirzapourdelavar H, Aleshin S, Schott BH, Seidenbecher C, Kaushik R, Dityatev A
Matrix biology : journal of the International Society for Matrix Biology (2025) : . 386 004 WB, ICC; tested species: mouse

Postsynaptic β1 spectrin maintains Na+ channels at the neuromuscular junction.
Sert O, Ding X, Zhang C, Mi R, Hoke A, Rasband MN
The Journal of physiology (2024) 6026: 1127-1145. 386 004 WB; tested species: mouse

Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG.
Ding X, Wu Y, Vainshtein A, Rodriguez V, Ricco E, Okoh JT, Liu Y, Kraushaar DC, Peles E, Rasband MN
Nature communications (2024) 151: 10865. 386 004 WB, IHC; tested species: mouse

Membrane mechanics dictate axonal pearls-on-a-string morphology and function.
Griswold JM, Bonilla-Quintana M, Pepper R, Lee CT, Raychaudhuri S, Ma S, Gan Q, Syed S, Zhu C, Bell M, Suga M, et al.
Nature neuroscience (2024) : . 386 004 ICC; tested species: mouse

Transcriptomic alterations in APP/PS1 mice astrocytes lead to early postnatal axon initial segment structural changes.
Benitez MJ, Retana D, Ordoñez-Gutiérrez L, Colmena I, Goméz MJ, Álvarez R, Ciorraga M, Dopazo A, Wandosell F, Garrido JJ
Cellular and molecular life sciences : CMLS (2024) 811: 444. 386 004 ICC; tested species: mouse

Immunoproximity biotinylation reveals the axon initial segment proteome.
Zhang W, Fu Y, Peng L, Ogawa Y, Ding X, Rasband A, Zhou X, Shelly M, Rasband MN, Zou P
Nature communications (2023) 141: 8201. 386 004 ICC, IHC; tested species: rat

Disruption of tubulin-alpha4a polyglutamylation prevents aggregation of hyper-phosphorylated tau and microglia activation in mice.
Hausrat TJ, Janiesch PC, Breiden P, Lutz D, Hoffmeister-Ullerich S, Hermans-Borgmeyer I, Failla AV, Kneussel M
Nature communications (2022) 131: 4192. 386 004 ICC; tested species: mouse

The amyloid precursor protein is a conserved Wnt receptor.
Liu T, Zhang T, Nicolas M, Boussicault L, Rice H, Soldano A, Claeys A, Petrova I, Fradkin L, De Strooper B, Potier MC, et al.
eLife (2021) 10: . 386 004 ICC; tested species: mouse

NuMA1 promotes axon initial segment assembly through inhibition of endocytosis.
Torii T, Ogawa Y, Liu CH, Ho TS, Hamdan H, Wang CC, Oses-Prieto JA, Burlingame AL, Rasband MN
The Journal of cell biology (2020) 2192: . 386 004 ICC; KD verified; tested species: mouse

TRIM46 organizes microtubule fasciculation in the axon initial segment.
Harterink M, Vocking K, Pan X, Soriano Jerez EM, Slenders L, Fréal A, Tas RP, van de Wetering WJ, Timmer K, Motshagen J, van Beuningen SFB, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 386 004 ICC; tested species: rat

The awakening of dormant neuronal precursors in the adult and aged brain.
Benedetti B, Reisinger M, Hochwartner M, Gabriele G, Jakubecova D, Benedetti A, Bonfanti L, Couillard-Despres S
Aging cell (2023) : e13974. 386 004 IHC; tested species: mouse

Chronic partial TrkB activation reduces seizures and mortality in a mouse model of Dravet syndrome.
Gu F, Parada I, Yang T, Longo FM, Prince DA
Proceedings of the National Academy of Sciences of the United States of America (2022) 1197: . 386 004 IHC; tested species: mouse

Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3.
Baidoe-Ansah D, Sakib S, Jia S, Mirzapourdelavar H, Strackeljan L, Fischer A, Aleshin S, Kaushik R, Dityatev A
Cells (2022) 1113: . 386 004 IHC; tested species: mouse

Morphological properties of the axon initial segment-like process of AII amacrine cells in the rat retina.
Liu JH, Singh JB, Veruki ML, Hartveit E
The Journal of comparative neurology (2021) 52916: 3593-3620. 386 004 IHC; tested species: rat

Granule Cell Ensembles in Mouse Dentate Gyrus Rapidly Upregulate the Plasticity-Related Protein Synaptopodin after Exploration Behavior.
Paul MH, Choi M, Schlaudraff J, Deller T, Del Turco D
Cerebral cortex (New York, N.Y. : 1991) (2019) : . 386 004 IHC; tested species: mouse

An ankyrin G-binding motif mediates TRAAK periodic localization at axon initial segments of hippocampal pyramidal neurons.
Luque-Fernández V, Vanspauwen SK, Landra-Willm A, Arvedsen E, Besquent M, Sandoz G, Rasmussen HB
Proceedings of the National Academy of Sciences of the United States of America (2024) 12131: e2310120121. 386 004 IHC-Fr; tested species: rat

Cat. No.: 386 004

Amount: 100 µl

Price: $375.00

References
WB (3)
ICC (8)
IHC (7)
IHC-Fr (1)

ICC

IHC

IHC-P

Background

Ankyrin G is a membrane-cytoskeleton linker. It may participate in the targeting and clustering of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (AIS).

1 ) Fujiwara Y et al., Sci Rep (2016)
2 ) Yang Y et al., J. Cell Biol. (2007)
3 ) Zhou D et al., J. Cell Biol. (1998)
4 ) Wood SJ et al., J. Cell Biol. (1998)
5 ) Kordeli E et al., J. Biol. Chem. (1995)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

Other products for this target

Ankyrin G antibody - 386 004 K.D.

References for Ankyrin G - 386 004