Synaptic Systems - Ankyrin G

Cat. No.: 386 003

Amount: 50 µg

Price: $385.00

Cat. No. 386 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Ankyrin G. (UniProt Id: G5E8K5-1)
Reactivity	Reacts with: rat (O70511-1), mouse (G5E8K5-1), human (Q12955). Other species not tested yet.
Specificity	Specific for Ankyrin G, detects all described splice variants.
Data sheet	386_003.pdf

References for Ankyrin G - 386 003

WB (1)
ICC (9)
IHC (6)

Impaired OTUD7A-dependent Ankyrin regulation mediates neuronal dysfunction in mouse and human models of the 15q13.3 microdeletion syndrome.
Unda BK, Chalil L, Yoon S, Kilpatrick S, Irwin C, Xing S, Murtaza N, Cheng A, Brown C, Afonso A, McCready E, et al.
Molecular psychiatry (2023) 284: 1747-1769. 386 003 WB, ICC; tested species: human,mouse

Altered neurological and neurobehavioral phenotypes in a mouse model of the recurrent KCNB1-p.R306C voltage-sensor variant.
Kang SK, Hawkins NA, Thompson CH, Baker EM, Echevarria-Cooper DM, Barse L, Thenstedt T, Dixon CJ, Speakes N, George AL, Kearney JA, et al.
Neurobiology of disease (2024) 194: 106470. 386 003 ICC; tested species: mouse

Neurofilament Levels in Dendritic Spines Associate with Synaptic Status.
Gürth CM, do Rego Barros Fernandes Lima MA, Macarrón Palacios V, Cereceda Delgado AR, Hubrich J, D'Este E
Cells (2023) 126: . 386 003 ICC; tested species: rat

Spectrin-beta 2 facilitates the selective accumulation of GABAA receptors at somatodendritic synapses.
Smalley JL, Cho N, Ng SFJ, Choi C, Lemons AHS, Chaudry S, Bope CE, Dengler JS, Zhang C, Rasband MN, Davies PA, et al.
Communications biology (2023) 61: 11. 386 003 ICC; tested species: mouse

Endocytosis in the axon initial segment maintains neuronal polarity.
Eichel K, Uenaka T, Belapurkar V, Lu R, Cheng S, Pak JS, Taylor CA, Südhof TC, Malenka R, Wernig M, Özkan E, et al.
Nature (2022) : . 386 003 ICC; tested species: mouse

The core PCP protein Prickle2 regulates axon number and AIS maturation by binding to AnkG and modulating microtubule bundling.
Dorrego-Rivas A, Ezan J, Moreau MM, Poirault-Chassac S, Aubailly N, De Neve J, Blanchard C, Castets F, Fréal A, Battefeld A, Sans N, et al.
Science advances (2022) 836: eabo6333. 386 003 ICC; tested species: rat

Neuregulins 1, 2, and 3 Promote Early Neurite Outgrowth in ErbB4-Expressing Cortical GABAergic Interneurons.
Rahman-Enyart A, Lai C, Prieto AL
Molecular neurobiology (2020) : . 386 003 ICC; tested species: rat

Phosphorylation on Ser 359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses.
Nakamura Y, Morrow DH, Nathanson AJ, Henley JM, Wilkinson KA, Moss SJ
The Journal of biological chemistry (2020) : . 386 003 ICC; tested species: rat

Loss of Neurofascin-186 Disrupts Alignment of AnkyrinG Relative to Its Binding Partners in the Axon Initial Segment.
Alpizar SA, Baker AL, Gulledge AT, Hoppa MB
Frontiers in cellular neuroscience (2019) 13: 1. 386 003 ICC; tested species: rat

Visual activity enhances neuronal excitability in thalamic relay neurons.
Duménieu M, Fronzaroli-Molinieres L, Naudin L, Iborra-Bonnaure C, Wakade A, Zanin E, Aziz A, Ankri N, Incontro S, Denis D, Marquèze-Pouey B, et al.
Science advances (2025) 114: eadp4627. 386 003 IHC; tested species: rat

Development of myelination and axon diameter for fast and precise action potential conductance.
Nabel AL, Teich L, Wohlfrom H, Alexandrova O, Heß M, Pecka M, Grothe B
Glia (2024) : . 386 003 IHC

Motor learning changes the axon initial segment of the spinal motoneuron.
Wang Y, Chen Y, Chen L, Herron BJ, Chen XY, Wolpaw JR
The Journal of physiology (2024) 6029: 2107-2126. 386 003 IHC; tested species: rat

Kv7/KCNQ potassium channels in cortical hyperexcitability and juvenile seizure-related death in Ank2-mutant mice.
Oh H, Lee S, Oh Y, Kim S, Kim YS, Yang Y, Choi W, Yoo YE, Cho H, Lee S, Yang E, et al.
Nature communications (2023) 141: 3547. 386 003 IHC; tested species: mouse

Development of microglia in fetal and postnatal neocortex of the pig, the European wild boar (Sus scrofa).
Sobierajski E, Lauer G, Aktas M, Beemelmans C, Beemelmans C, Meyer G, Wahle P
The Journal of comparative neurology (2021) : . 386 003 IHC; tested species: pig

Deletion of class II ARFs in mice causes tremor by the Nav1.6 loss in cerebellar Purkinje cell axon initial segments.
Hosoi N, Shibasaki K, Hosono M, Konno A, Shinoda Y, Kiyonari H, Inoue K, Muramatsu SI, Ishizaki Y, Hirai H, Furuichi T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 386 003 IHC; tested species: mouse

Cat. No.: 386 003

Amount: 50 µg

Price: $385.00

References
WB (1)
ICC (9)
IHC (6)

Development of myelination and axon diameter for fast and precise action potential conductance.
Nabel AL, Teich L, Wohlfrom H, Alexandrova O, Heß M, Pecka M, Grothe B
Glia (2024) : . 386 003 IHC

ICC

IHC

IHC-P

Background

Ankyrin G is a membrane-cytoskeleton linker. It may participate in the targeting and clustering of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (AIS).

1 ) Fujiwara Y et al., Sci Rep (2016)
2 ) Yang Y et al., J. Cell Biol. (2007)
3 ) Zhou D et al., J. Cell Biol. (1998)
4 ) Wood SJ et al., J. Cell Biol. (1998)
5 ) Kordeli E et al., J. Biol. Chem. (1995)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

Other products for this target

Ankyrin G antibody - 386 003

References for Ankyrin G - 386 003