Cat. No.: 372 011C2
Amount: 100 µg
Price:
$465.00
Cat. No. 372 011C2 |
100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2.
Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.
(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: N/A Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not tested yet Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.', $event)" style="cursor: help;">FACS: yes (see remarks) |
Label | Cyanine 2 |
Clone | 164G11 |
Subtype | IgG2a (κ light chain) |
Immunogen | Purified human basophile granulocytes. |
Reactivity |
Reacts with: human. Other species not tested yet. |
Remarks |
FACS: This antibody does not activate basophiles upon binding. |
Data sheet | 372_011c2.pdf |