Synaptic Systems

Cat. No.: 371 011C2

Amount: 100 µg

Price: $470.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

Background

CD63, also known as melanoma-associated antigen ME491, is a member of the tetraspanin superfamily, which is characterized by proteins containing four transmembrane domains (1). CD63 is extensively N-glycosylated and is predominantly localized to late endosomes, lysosomes, secretory vesicles, and the plasma membrane (1–3). It is ubiquitously expressed in normal tissues and is markedly upregulated in activated basophils and mast cells, where it is required for efficient IgE-mediated degranulation (4,5).
In melanoma, CD63 is highly expressed during early stages of disease, where it has been implicated in the suppression of epithelial-to-mesenchymal transition (EMT); however, its expression is reduced in more advanced lesions (6). In addition, CD63 expression has been reported to possess prognostic significance in several other solid tumors (7).

1 ) Metzelaar MJ et al., J. Biol. Chem. (1991)
2 ) Escola JM et al., J Biol Chem (1998)
3 ) Duffield A et al., Proc Natl Acad Sci U S A (2003)
4 ) Kraft S et al., J. Immunol. (2013)
5 ) Tammaro A et al., Int J Immunopathol Pharmacol ()
6 ) Lupia A et al., J. Invest. Dermatol. (2014)
7 ) Koh HM et al., In Vivo (2020)

Protocols

ICC

Fixed Cells

Other products for this target

Cat. No. 371 011C2	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: not recommended IP: N/A ICC: 1 : 250 up to 1 : 1000 gallery IHC: not tested yet IHC-P (FFPE): not tested yet FACS: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Label	Cyanine 2
Clone	101G9
Subtype	IgG2b (κ light chain)
Immunogen	Purified human basophil granulocytes. (UniProt Id: P08962)
Epitop	AA 103 to 203 from human CD63 (UniProt Id: P08962)
Reactivity	Reacts with: human (P08962). Other species not tested yet.
Data sheet	Data Sheet 371_011c2 Safety Data Sheet (SDS)

CD63 antibody - 371 011C2