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Home > Products > 369 003

DOPA decarboxylase antibody - 369 003

DOPA decarboxylase is an enzyme for dopamine, serotonin, and trace amine biosynthesis
Rabbit polyclonal purified antibody
Cat. No.: 369 003
Amount: 50 µg
Price: $375.00
Cat. No. 369 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 up to 1 : 5000 gallery  
IHC-P: 1 : 500 up to 1 : 5000     gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 480 from mouse DDC (UniProt Id: O88533)
Reactivity Reacts with: rat (P14173), mouse (O88533).
Other species not tested yet.
Data sheet 369_003.pdf

References for DOPA decarboxylase - 369 003

Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 369 003 WB, IHC; tested species: mouse
β-synuclein potentiates synaptic vesicle dopamine uptake and rescues dopaminergic neurons from MPTP-induced death in the absence of other synucleins.
Ninkina N, Millership SJ, Peters OM, Connor-Robson N, Chaprov K, Kopylov AT, Montoya A, Kramer H, Withers DJ, Buchman VL
The Journal of biological chemistry (2021) : 101375. 369 003 WB; tested species: mouse
Loss of PTEN-induced kinase 1 (Pink1) reduces hippocampal tyrosine hydroxylase and impairs learning and memory.
Maynard ME, Redell JB, Kobori N, Underwood EL, Fischer TD, Hood KN, LaRoche V, Waxham MN, Moore AN, Dash PK
Experimental neurology (2019) 323: 113081. 369 003 WB; tested species: mouse
Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 369 003 WB, IHC; tested species: mouse
Cat. No.: 369 003
Amount: 50 µg
Price: $375.00
Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 369 003 WB, IHC; tested species: mouse
β-synuclein potentiates synaptic vesicle dopamine uptake and rescues dopaminergic neurons from MPTP-induced death in the absence of other synucleins.
Ninkina N, Millership SJ, Peters OM, Connor-Robson N, Chaprov K, Kopylov AT, Montoya A, Kramer H, Withers DJ, Buchman VL
The Journal of biological chemistry (2021) : 101375. 369 003 WB; tested species: mouse
Loss of PTEN-induced kinase 1 (Pink1) reduces hippocampal tyrosine hydroxylase and impairs learning and memory.
Maynard ME, Redell JB, Kobori N, Underwood EL, Fischer TD, Hood KN, LaRoche V, Waxham MN, Moore AN, Dash PK
Experimental neurology (2019) 323: 113081. 369 003 WB; tested species: mouse
Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 369 003 WB, IHC; tested species: mouse
WB
IHC
IHC
IHC
IHC-P
IHC-P
Background

DOPA decarboxylase, also referred to as aromatic L-amino acid decarboxylase, AADC or DDC, is the second enzyme in the biosynthesis of dopamine and serotonin. In addition, it is responsible for the synthesis of the trace amines phenylethylamine, p-tyramine, and tryptamine, which are considered to act as neuromodulators.
DDC is expressed in the central nervous system, but has also been detected in several tissues such as liver, kidney, small intestine, adrenal gland and blood vessels.
The inhibition of DDC is used for the treatment of Parkinson's disease.
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