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Glutamine synthetase - 367 011

An enzyme for glutamine synthesis and ammonia detoxification, a marker for astrocytes
Monoclonal mouse purified IgG
Cat. No.: 367 011
Amount: 100 µg
Price: $415.00
Cat. No. 367 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 up to 1 : 2000 gallery  
IHC-P/FFPE: 1 : 5000 up to 1 : 20000 gallery  
Clone 250C4
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 2 to 373 from mouse Glutamine synthetase (UniProt Id: P15105)
Reactivity Reacts with: rat (P09606), mouse (P15105).
Other species not tested yet.
Specificity Specific for Glutamine synthetase
Data sheet 367_011.pdf
Cat. No.: 367 011
Quantity: 100 µg
Price: $415.00
Background
Glutamine synthetase, also referred to as Glutamate-ammonia ligase or GS, is an enzyme that catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. It is present predominantly in brain, kidneys, and liver. In the brain, it is particularly found in astrocytes.
Glutamine synthetase plays a pivotal role in glutamate and glutamine homoeostasis, and it is largely responsible for the removal of both blood-derived and metabolically generated ammonia, preventing neurotoxicity. It is also a key enzyme in the recycling of the neurotransmitter glutamate.
Several studies indicated that the expression, distribution, and activity of brain glutamine synthetase is altered in some brain disorders, including Alzheimer's disease, schizophrenia, depression, suicidality, and mesial temporal lobe epilepsy (MTLE).