Synaptic Systems - Active Zone

Cat. No.: 364 003

Amount: 50 µg

Price: $375.00

Cat. No. 364 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not recommended ICC: 1 : 500 gallery IHC: not tested yet IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 466 from rat Rim2 (UniProt Id: Q9JIS1)
Reactivity	Reacts with: rat (Q9JIS1), mouse. Other species not tested yet.
Specificity	Detects Rim 1, Rim 2 and Piccolo. Antiserum has been affinity purified with a synthetic peptide corresponding to aa 41-59 in mouse Piccolo. This epitope is conserved in Rim 1,
Data sheet	364_003.pdf

References for Active Zone - 364 003

Homozygous Expression of Mutant ELOVL4 Leads to Seizures and Death in a Novel Animal Model of Very Long-Chain Fatty Acid Deficiency.
Hopiavuori BR, Deák F, Wilkerson JL, Brush RS, Rocha-Hopiavuori NA, Hopiavuori AR, Ozan KG, Sullivan MT, Wren JD, Georgescu C, Szweda L, et al.
Molecular neurobiology (2018) 552: 1795-1813. 364 003 ICC; tested species: mouse

Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses.
Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, Peukert A, Martens H, Feigenspan A, Kremers J, Schoch S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7848-7863. 364 003 ICC, IHC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 364 003 DNA-PAINT; tested species: rat

Cat. No.: 364 003

Amount: 50 µg

Price: $375.00

ICC

IHC-P

Background

The active zone is the region in the presynaptic nerve terminal that mediates neurotransmitter release. It consists of the presynaptic membrane and a dense protein structure called the cytomatrix at the active zone (CAZ).

1 ) Gundelfinger ED et al., Front Synaptic Neurosci (2015)
2 ) Szule JA et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. (2015)
3 ) Michel K et al., Exp. Cell Res. (2015)

Protocols

ICC

IHC-P

Active Zone antibody - 364 003

References for Active Zone - 364 003