Neurogranin antibody - 357 003

Neurogranin is a postsynaptic substrate for PKC

Rabbit polyclonal purified antibody

Cat. No.: 357 003

Amount: 50 µg

Price: $375.00

Cat. No. 357 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 up to 1 : 2000 gallery IHC: 1 : 1000 up to 1 : 2000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 57 to 78 from rat Neurogranin (UniProt Id: Q04940)
Reactivity	Reacts with: rat (Q04940), mouse (P60761). Other species not tested yet.
Data sheet	357_003.pdf

References for Neurogranin - 357 003

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 357 003 ICC; tested species: rat

Cat. No.: 357 003

Amount: 50 µg

Price: $375.00

ICC

IHC

Background

Neurogranin is a postsynaptic substrate for protein kinase C (PKC) that is related to postsynaptic plasticity. It is exclusively expressed in the brain where it is enriched at dendritic spines.

1 ) Huang KP et al., ACS Chem Neurosci (2011)
2 ) Zhong L et al., EMBO J. (2009)
3 ) Zhabotinsky AM et al., J. Neurosci. (2006)
4 ) Higo N et al., Cereb. Cortex (2004)

Protocols

ICC

IHC