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CNP1 antibody - 355 006 K.O.

CNP1 is a marker for oligodendrocytes
Chicken polyclonal purified antibody
Cat. No.: 355 006
Amount: 50 µg
Price: $385.00
Cat. No. 355 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 AP staining gallery  
ICC: 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 4000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 1 to 420 from mouse CNP1 (UniProt Id: P16330)
Reactivity Reacts with: rat (P13233), mouse (P16330).
Other species not tested yet.
Specificity K.O. validated
Remarks

ICC: Methanol fixation is recommended.
IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended.

Data sheet 355_006.pdf

References for CNP1 - 355 006

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 355 006 IHC; tested species: mouse
Cat. No.: 355 006
Amount: 50 µg
Price: $385.00
INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 355 006 IHC; tested species: mouse
Background
The 2', 3'-cyclic nucleotide 3'-phosphodiesterase CNP 1, also referred to as CNPase and CNP, is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system.
It is assumed that CNP 1 participates in RNA metabolism of myelinating oligodendrocytes.