CD31 antibody - 351 011

CD31 is an endothelial cell marker

Mouse monoclonal purified IgG

Cat. No.: 351 011

Amount: 100 µg

Price: $420.00

Cat. No. 351 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not recommended FACS: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Clone	10F7
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 26 to 601 from human CD31 (UniProt Id: P16284)
Reactivity	Reacts with: human (P16284). Other species not tested yet.
Specificity	Recognizes all CD 31 isoforms.
Data sheet	351_011.pdf

References for CD31 - 351 011

Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.
Mohr F, Przibilla S, Leonhardt F, Stemberger C, Dreher S, Müller TR, Fräßle SP, Schmidt GP, Kiene ML, Stadler H, Busch DH, et al.
Scientific reports (2018) 81: 16731. 351 011 FACS; tested species: human

Cat. No.: 351 011

Amount: 100 µg

Price: $420.00

ICC

Background

CD31 or platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a cell adhesion molecule that contains a single trans-membrane domain and 6 Ig-like C2-type (immunoglobulin-like) domains (1). It is expressed on the surface of leukocytes and platelets and is primarily concentrated at endothelial cell-cell junctions (2). CD31 plays a role in several important biological processes, such as vascular development, maintenance of vascular endothelial barrier function and leukocyte emigration at inflammatory sites. In addition, CD31 promotes cancer metastasis by inducing epithelial-mesenchymal transition (3).

1 ) Privratsky JR et al., Cell Tissue Res. (2014)
2 ) Lertkiatmongkol P et al., Curr Opin Hematol (2016)
3 ) Zhu X et al., Gut Liver (2021)

Protocols

ICC

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