Tryptophan hydroxylase2 antibody - 348 011 K.O.

Tryptophan hydroxylase2 is an enzyme involved in serotonin synthesis

Mouse monoclonal purified IgG

Cat. No.: 348 011

Amount: 100 µg

Price: $420.00

Cat. No. 348 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	269E7
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to the N-terminal part of rat TPH2. (UniProt Id: Q8CGU9)
Reactivity	Reacts with: rat (Q8CGU9), mouse (Q8CGV2). Other species not tested yet.
Specificity	K.O. validated
Remarks	IHC: This antibody gives reliable labeling on vibratome‑cut sections, but its sensitivity drops when applied to cryostat sections of PFA‑fixed tissue. We recommend our rabbit polyclonal antibody (cat. no. 348 003) for better performance on frozen sections.
Data sheet	Datasheet 348_011

References for Tryptophan hydroxylase2 - 348 011

A Squeak Is Not Enough: Female Presence and Vocal Playback Have Contrasting Effects on c-Fos Expression by Dorsal Raphé Neurons in Lab Mice.
Walker M, Bush J, Hurley LM
Brain sciences (2026) 162: . 348 011 IHC; tested species: mouse

Cat. No.: 348 011

Amount: 100 µg

Price: $420.00

IHC

IHC-P

Background

Tryptophan hydroxylase (TPH) is an enzyme that catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin).
Two isoforms TPH1 and TPH2 have been described. TPH1 occurs mainly in tissues that express serotonin in the periphery (skin, gut, pineal gland). TPH2 is exclusively expressed in neuronal cell types and is the predominant isoform in the central nervous system.
In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain.

1 ) Chen GL et al., Biochem. Pharmacol. (2013)
2 ) Martínez A et al., Curr. Med. Chem. (2001)
3 ) Garber SL et al., Brain Res. (1987)

Protocols

IHC

IHC-P

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